摘要
应用高效液相色谱法测定了染发剂中2-硝基-p-苯二胺的含量。样品用乙醇-水(1+1)混合溶液提取后,经水系C18色谱柱分离,以(A)10mol·L-1柠檬酸缓冲溶液-10mol·L-1辛烷磺酸钠溶液(pH 2.6)与(B)乙腈以体积比75比25组成的混合溶液为流动相进行等度洗脱,用二极管阵列检测器于410nm检测。2-硝基-p-苯二胺的线性范围为2.4～240mg·L-1,检出限(3S/N)为0.7mg·L-1。以染发剂样品为基体,进行加标回收试验,回收率在90.4%～99.4%之间,相对标准偏差(n=6)在0.35%～3.0%之间。
HPLC was applied to the determination of 2-nitro-p-benzenediamine in hair dyes. The sample was extracted with mixture of CHaCH2OH-H20 （1＋1）. The extract was separated on auqo-C18 column by gradient elution with eluants containing A （10 mol · L^-1 citric acid ＋10 mol · L^-1 sodium octanesulfonate of pH 2. 6） and B （acetonitrile） mixed in the ratio of 75 to 25, and determined with diode array detector at the wavelengths of 410 nln. Linearity range for 2-nitro-p-benzenediamine was found between 2.4--240 mg · L^-1, with detection limit （3S/N） of 0. 7 mg · L^-1. Using sample of hair dyes as matrix, recovery test was made by standard addition method, values of recovery found were in the range of 90. 4%-99.4%, with RSD＇s （n=6） in the range of 0. 35%-3.0%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2012年第2期185-187,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
厦门出入境检验检疫局科技基金(2007XK03)
