摘要
目的:观察抑郁大鼠脑内细胞外信号调节激酶(ERK)通路的表达及颐脑解郁方的干预作用。方法:建立抑郁模型,采用蛋白印迹(Western Blot)实验方法,研究ERK1/2在抑郁模型大鼠脑内左前额叶皮质、海马的表达及颐脑解郁方的干预作用。结果:模型组大鼠海马、前额皮质的ERK1/2表达明显下降,与正常组相比较,有显著性差异(P<0.05,P<0.01);颐脑解郁方干预后可上调ERK1/2在脑内的表达,与模型组相比有显著性差异(P<0.05,P<0.01)。结论:抑郁模型大鼠脑内ERK1/2表达降低,颐脑解郁方可使之明显上调,颐脑解郁方的抗抑郁作用可能与上调ERK1/2信号转导通路有关。
Objective： To observe the expression of ERK1/2 and interference effect of Yinao Jieyu Recipe on the rat model of depression.Methods： Western Blot was used in the rat model of depression.The expression of ERK1/2 in prefrontal cortex and hippocampus,and the interference of Yinao Jieyu Recipe were observed.Results： The expression of ERK1/2 in hippocampus and prefrontal cortex in groups of the model of depression decreased obviously compared to that of the normal group（P〈0.05,P〈0.01）.Yinao Jieyu Recipe enhanced obviously the expression of ERK1/2（P〈0.05,P〈0.01）.Conclusion： The expression of ERK1/2 in the brain of depression rat model decreased,while Yinao Jieyu Recipe can enhance this expression.The results showed that the anti-depression effect of Yinao Jieyu Recipe may be related to its function of increasing expression of ERK1/2 signal transduction.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2012年第3期619-621,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
教育部高等学校博士学科点专项科研基金资助项目(No.707009)~~
