摘要
目的检测外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)中CD161+T细胞的频数变化,探讨其在类风湿关节炎(RA)发生、发展中的作用。方法收集唐山市工人医院风湿病科2009年6月至2010年6月21例类风湿关节炎患者,其中男性7例,女性14例,平均年龄55岁。采用PBMCs体外活化技术,应用流式细胞术检测RA患者外周血PBMCs中CD4+CD161+T细胞的频数情况。结果与年龄、性别匹配的健康人群相,RA患者PBMCs中CD161+CD4+T和CD161+Th1细胞的百分比显著降低[(27.53±7.34)%vs(22.75±5.11)%,(P<0.05);(11.35±4.36)%vs(8.21±3.93)%,(P<0.05)],导致RA患者CD161+与CD161-Th1细胞的比率下降,二者差异有显著性[(0.76±0.29)%vs(0.60±0.30)%,(P<0.05)]。结论 RA患者滑膜炎性反应导致外周血CD161+CD4+T细胞及其Th1亚群与相应阴性T细胞群体的平衡紊乱,CD161+CD4+T细胞及其Th1亚群在RA的病理过程密切相关。
Objective To investigate the frequency of CD161＋ T cells in peripheral blood mononuclear cells（PBMCs） from patients with rheumatoid arthritis（RA）.Methods A total of 21 patients diagnosed as RA（14 women and 7 men,at an average age of 55） who hospitalized in our department from June 2009 to June 2010 were enrolled in this study.Another 21 age-and sex-matched healthy volunteers served as normal control.PBMCs were obtained from RA patients and healthy subjects.CD161 positive T cells were identified by a four-color flow cytometer after ex vivo stimulation with phorbol myristate acetate and ionomycin in the present of brefeldin A（BFA）.Results The frequencies of CD161＋ T cells and CD161＋ Th1 cells were significantly decreased within CD4＋ T cells of PBMCs from RA patients,respectively [（27.53±7.34）% vs（22.75±5.11）%,P0.05;（11.35±4.36）% vs（8.21±3.93）%,P0.05],which led to a decreased percentage of CD161＋ and CD161-Th1 cells in RA patients.Conclusion The inflammatory response of synovial membrane in RA patients results in the changes of CD161＋CD4＋ T cells and CD161＋ Th1 cells,rather than those of the corresponding CD161-T cells,which suggests CD161＋ T cells might play an important role in the development of RA.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2012年第5期438-441,共4页
Journal of Third Military Medical University
