摘要
本研究旨在探明鼠李糖乳杆菌(L.rhamnosus)对Caco-2细胞抗氧化和非特异免疫功能的影响。Caco-2细胞分别用PBS(A组)、E.coli K88(B组)和L.rhamnosus(C组)处理,以及先用L.rhamnosus预处理,再用E.coli K88处理(D组)。结果表明,B组细胞上清的T-AOC浓度显著降低(P<0.01),SOD活力显著提高(P<0.01),C组与此相反,并且细胞裂解液中的SOD活性显著提高(P<0.05)。B组和C组不同程度地促进APRIL的分泌(P<0.01),而抑制IL-8的产生(P<0.01)。B组也抑制IL-10的产生,但C组却显著促进其分泌(P<0.01)。与B组相比,D组APRIL显著减少(P<0.01),但IL-8和IL-10都显著增加(P<0.05,P<0.01)。结果提示,L.rhamnosus可提高Caco-2细胞的抗氧化能力,减轻炎症反应,具有保护作用和免疫佐剂的效果。
In this study, the effects of Lactobacillus rhamnosus on anti-oxidative and non-specific immunologic functions in Caco-2 cells were investigated. Caco-2 cells were treated by PBS（group A）, E. coli K88（group B） and L. rharnnosus （group C）, respectively. Caco-2 cells in group D were pre-incubated with L. rharnnosus and then infected with E. coli K88. The results showed that T-AOC concentration decreased（P〈0.01）, but SOD activity enhanced in cell culture super- natant（P〈0.01）in group B. However, the adverse situation appeared in group C and, SOD activ- ity in cell lysate increased（P〈0.05）. APRIL secretion were induced（P〈0.01）, however, IL-8 secre- tion were inhibited（P〈0.01）both in group B and group C in different degree. IL-10 production was stopped in group B, and was promoted in group C（P〈0.01）. Compared with group B, A- PRIL production significantly decreased（P〈0.01）, whereas levels of IL-8 （P〈0.05）and IL-10 （P〈0.01）increased in group D. These results suggested that Caco-2 cells were sensitize to re- spond to L. rharnnosus by an improved response in anti-oxidative function. In addition, inflam- matory response against pathogenic bacteria in enterocyte attenuated, moreover, immune re- sponse reinforced, which indicated the activities for immunoprotection and immunoadjuvant in L.rhamnosus,
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2012年第2期250-254,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
浙江省重大科技专项(2006C12086)
国家重点基础研究发展计划(2009CB118705)
博士学科点专项科研基金(20110101110101)
