摘要
背景:有研究表明胰岛素样生长因子1和血小板源性生长因子可抑制人椎间盘细胞凋亡。目的:观察在体外培养条件下胰岛素样生长因子1、血小板源性生长因子对人退变髓核细胞生物学活性的影响。方法:体外单层培养人退变髓核细胞,通过免疫组织化学鉴定细胞。对传3代人退变髓核细胞采用分别不同生长因子干预,实验分4组:胰岛素样生长因子1组,血小板源性生长因子组,胰岛素样生长因子1+血小板源性生长因子组及对照组。结果与结论:胰岛素样生长因子1、血小板源性生长因子均可促进细胞增殖,促进细胞合成Ⅱ型胶原和聚集蛋白聚糖,其中胰岛素样生长因子1促Ⅱ型胶原合成作用强于血小板源性生长因子(P<0.05),血小板源性生长因子促蛋白聚糖合成作用强于胰岛素样生长因子1(P<0.05)。胰岛素样生长因子1促进细胞合成Ⅰ型胶原,血小板源性生长因子抑制细胞合成Ⅰ型胶原。结果证实,胰岛素样生长因子1、血小板源性生长因子均可通过促进细胞增殖、促进细胞合成Ⅱ型胶原和聚集蛋白聚糖,从而提高人退变髓核细胞的生物学活性。
BACKGROUND： Previous studies showed that insulin-like growth factor-1 （IGF-1） and platelet-derived growth factor （PDGF） can suppress apoptosis of human intervertebral cells. OBJECTIVE： To observe the effects of IGF-1 and PDGF on the biological potential of the human degenerative NP cells in vitro. METHODS： Human degenerative nucleus pulposus cells were cultured in monolayer in vitro and identified by immunohistochemical staining. The cells of passage 3 were treated separately with different growth factors and then randomly divided into 4 groups： IGF-1 group, PDGF group, IGF-1＋PDGF group and control group. RESULTS AND CONCLUSION： IGF-1 and PDGF could stimulate cell proliferation and synthesis of collagen type Ⅱ and aggrecan, and IGF-1 raised more content of collagen typeⅡ than the PDGF （P 0.05）, but the PDGF raised more content of aggrecan than the IGF-1 （P 0.05）. IGF-1 stimulated the synthesis of collagen typeⅠ, then PDGF inhibited the synthesis. Both the IGF-1 and PDGF can stimulate cell proliferation, the synthesis of collagen type Ⅱ and aggrecan, thereby enhance the biological potential of the human degenerative nucleus pulposus cells.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
2012年第2期223-226,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
