摘要
目的探讨Survivin过量表达对MS1细胞Survivin基因及蛋白表达与凋亡的影响。方法构建Survivin真核表达载体,以脂质体转染MS1细胞,采用流式细胞仪的方法检测细胞凋亡;Real-time PCR和Western blot检测Survivin的mRNA及蛋白水平。结果转染Survivin实验组与空载体对照组比较,Survivin mRNA和蛋白表达明显增高,实验组细胞凋亡率较对照组下降。结论 Survivin过表达可使MS1细胞的Survivin蛋白及mRNA高表达并能使细胞凋亡凋亡率下降,为后续的动物实验打下了理论依据。
Objective To investigate the effects of Survivin over expression on MS1 cell survivin mRNA and protein and cell apoptosis. Methods Transfecting Survivin gene into MS1 cell by Lipofectamine2000. The expression of Survivin mRNA and protein were detected respectively by Real time PCR and Western blot, Cell apoptosis was detected by Flow cytometric. Results Survivin expression was increased by transfecting Survivin gent into MS1, meanwhile, the cells apoptosis was reduced. Conclusion Survivin may reduce the cells apoptosis rate of MS1, the outcome of the experiment plays a good theoretical for the subsequent animal experiments.
出处
《世界科技研究与发展》
CSCD
2012年第1期140-142,共3页
World Sci-Tech R&D
