摘要
目的了解DPP梅毒筛查和确证检测试剂(胶体金法)与RPR和TPPA的差异。方法用DPP梅毒筛查和确证检测试剂分别检测高危人群245例全血、血浆、100例手指末梢血,同时245例高危人群的血清用RPR和TPPA方法检测。结果 DPP梅毒筛查和确证检测试剂检测全血、血浆和指血中的梅毒特异性抗体与TPPA比较,无显著性差异(χ2值0.25、1.5、0.0,P>0.05);全血、血浆和指血的非梅毒特异性抗体与RPR比较,无显著性差异(χ2值0.125、0.083、1.125,P>0.05)。DPP梅毒筛查和确证检测试剂的敏感性为94.3%～100%,特异性为78.6%～98.9%,准确性为92%～99%。结论 DPP梅毒筛查和确证检测试剂操作简单、快速,结果准确,可作为临床筛查和确诊梅毒的主要检测方法,值得推广应用。
Objective To investigate the differences between DPP syphilis screening and confirmation assay（colloidal gold method） and RPR,TPPA. Methods 245 cases of venous whole blood or plasma samples and 100 cases of finger-stick whole blood samples were detected with DPP syphilis screening and confirmation assay, and serum samples from the same 245 cases of subjects were detected with RPR and TPPA. Results There was no significant difference of syphilis specific antibody in venous whole blood, plasma and finger-stick whole blood samples, detected with DPP syphilis screening and confirmation assay and TPPA （X2 values were 0.25,1.5 and 0.0, P〉0.05）, and also between DPP syphilis screening and confirmation assay and RPR（X2 values were 0. 125,0. 083 and 1.125, P〉0.05）. The sensitivity, specificity and accuracy of DPP syphilis screening and confirmation assay were 94.3%- 100%,78. 6%- 98. 9% and 92% -99%. Conclusion DPP~ syphilis screen and confirm assay could effectively shorten testing time for the detection of syphilis,with fine accuracy,and could be used for the screening and confirmation of syphi- lis.
出处
《国际检验医学杂志》
CAS
2012年第4期399-400,403,共3页
International Journal of Laboratory Medicine
