摘要
目的建立一种便捷可靠的实验方法能够同时检测FLT3基因突变及NPMl基因突变。方法设计多重PCR结合毛细管电泳（CE）方法同时检测75例初诊急性白血病（AL）患者FLT3基因及NPMl基因突变发生情况。结果应用多重PCR结合CE方法在75例AL患者中检测到FLT3-ITD突变阳性28例，FLT3一TKD突变阳性5例，NPMl突变阳性20例，9例同时存在FLT3．ITD及NPMI基因突变，l例同时存在FLT3．ITD及FLT3．TKD突变。结论多重PCR结合cE是一种能同时检测FLT3基因和NPMl基因突变的便捷可靠的方法，对判断AML患者的临床预后、指导分层治疗有重要意义，有进一步推广的价值。
Objective To develop an assay to identify both FLT3 and Nucleophosmin（NPMl） gene mutations in a single multiplex polymerase chain reaction（PCR）.Methods Bone marrow mononuclear cells of 75 newly diagnosed acute leukemia（AL） patients were analyzed for both FLT3 and NPM1 mutations by a PCR and capillary electrophoresis（CE） assay.Results Of 75 AL samples analyzed,28 were FLT3-ITD positive,5 were FLT3- TKD positive,20 were NPMI positive.Among these patients with FLT3 or NPM1 mutations,one case was both FLT3-ITD and FLT3-TKD mutations,nine were both FLT3-ITD and NPM1 mutations.Conclusions Multiplex PCR followed by capillary electrophoresis can be used for a fast and reliable diagnosis of both FLT3 and NPM1 mutations.
出处
《中国血液流变学杂志》
CAS
2011年第4期569-572,共4页
Chinese Journal of Hemorheology
