摘要
目的 探讨β射线防治瘢痕增生的作用机理。 方法 用MTT法分析 β射线对增生性瘢痕成纤维细胞生长的影响 ;用电镜、DNA凝胶电泳和流式细胞仪观察细胞形态和DNA的变化。结果 β射线明显抑制增生性瘢痕成纤维细胞的生长 ;10Gyβ射线诱导出典型的细胞凋亡特征 ,如凋亡小体、DNA梯状条带、特征性亚G1峰等 ,2 0Gyβ射线导致细胞坏死。 结论 β射线诱导增生性瘢痕成纤维细胞凋亡是放射防治瘢痕增生的重要机理之一 。
Objective To investigate the role of β ray induced apoptosis of the fibroblasts on the inhibition of hypertrophic scar.Methods\ A series of patients with hypertrophic scar were grouply treated with a certain dose β ray.A liopic sample was taken in each patients.Its was then examined with MTT[3 (4,5 dimethylthiazol. 2 yl) 2,5 dipheny1 terazolium bromide]techinque for deciding the inhibition rate of the fibroblasts,with electron microscope technigque, DNA agarose gel electrophoresis and flow cytometry for evaluating the morphological and biochermical characteristics of the fibroblast.Results\ The β ray radiation resulted in significant inhibition of the fibroblast.The features of fibroblast apoptosis(e.g:apoptic bodies,DNA ladders band and hypodiploid DNA peak)could be seen in the group with lower does (10Gy).The features of fibroblast necrosis could also be found in the group with higher does(20Gy). Conclusions The β ray radiation could have the effect for inhibiting the proliferation of scar derived fibroblast,but with dose dependence.It could be used for the treatment and(or)the prevention of hypertrophic scar.
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2000年第2期69-72,共4页
Chinese Journal of Plastic Surgery
