摘要
【目的】探讨离断的坐骨神经 (sciaticnerve,SN)分泌的可溶性因子对培养中的成年青蛙背根神经节 (dorsalrootganglion ,DRG)神经突起生长及形态学的影响。【方法】分离成年青蛙DRG神经元培养于含有离断的SN条件性培养基(SNCM)中 ,检测SNCM促进神经突起生长的量效和时效作用及对生长的神经突起形态学的影响。【结果】在 0 1～ 5mg/L蛋白质量浓度范围内 ,SNCM促进神经突起生长的作用有蛋白质量浓度依赖关系。在培养的第 1天 ,实验组平均神经突起长度是对照组的 3 9倍 ,培养 3d后实验组神经元突起平均长度已达对照组神经元的 9 2倍 ,实验组和对照组两者有显著的差异 (P <0 0 0 1)。且实验组神经元生长出的突起缺乏对照组神经元那种典型的片层状伪足。活性因子的分子质量介于 30～10 0ku之间。【结论】离断的外周神经可释放可溶性的因子促进离体培养的成年DRG神经元突起的生长并影响突起的形态。
Objective To investigate the effects of diffusible factors secreted from the denervated sciatic nerve on the neurites growth and morphological changes of neurons from adult frog dorsal root ganglion (DRG) in the culture. Methods Neurons dissociated from adult frog DRG were cultured with the conditioned medium(CM) of denervated sciatic nerve. The average length of the processes of the cultured neurons was measured and the morphology of the neurons was examined after 1,3, and 5 days of culture. Results Between the range of 0 1～5 μg/ml,CM had an obvious effect in promoting the outgrowth of DRG neurites with a protein concentration dependent relation. The average process length of DRG neurons was 3 9 times that of the control neurons in the first day of culture and 9 2 times after 3 days in culture. The differences were significance between experiment and control groups (P<0 001). In addition, the neurites of DRG neurons in the experiments groups lacked lamellipodia that is typical on processes of control neurons. The molecular weigh of the active factors was between 30 ku to 100 ku. Conclusion The denervated peripheral nerve can release diffusible factors to promote the outgrowth and influence the morphology of neurites of DRG neurons in culture.
出处
《中山医科大学学报》
CSCD
2000年第2期92-95,共4页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
国家自然科学基金! ( 3 9970 2 3 7)
CMB!( 98677)
"2 11工程"资助!( 9814 2 )
关键词
坐骨神经
神经元
神经再生
背根节神经元
ganglion, spinal/drug effects
sciatic nerve/secretion
neuron/drug effects
nerve regeneration
