摘要
为了明确河南省洛阳地区牡丹叶部一种新病害的病原种类,对采自田间的典型症状叶片进行了常规组织分离,对单孢菌株进行了形态鉴定、rDNA ITS序列分析及致病性测定。结果表明,病原菌在PDA培养基上菌落初期白色绒毛状,后期红褐色。分生孢子梗通常呈扫帚状,有2～3个分支,末端膨大呈泡囊状。分生孢子直棒状,两端钝圆,有0～1个隔膜,大小为(38.4～86.4)μm×(4.6～5.9)μm,平均60.1μm×5.2μm。离体叶片与活体植株接种结果表明,该菌均能侵染牡丹叶片并引起与田间相同的症状。据此将分离物鉴定为Cylindrocladium cana-dense,将该病害命名为牡丹柱枝孢叶斑病。
In order to determine the pathogen of a new leaf spot of peony in Luoyang,Henan,the pathogen was isolated from the leaves with typical symptoms,and the strains of the single spore were identified with morphological characters and DNA sequence analysis,and tested according to the standard Koch＇s postulation methods.The results showed that on PDA,colony was white and orbicular,but gradually turned to red brown.Conidiophores had di-or trichotomic branches,the terminals of which expanded and formed a vesicle.Conidia were cylindrical,straight with rounded ends,with 0-1 dissepiments,smooth and（38.4-86.4）μm×（4.6-5.9）μm,and the average was 60.1 μm×5.2 μm.The results of inoculation on the leaves in petri dishes and in living peony bodies showed that the strains could infect peony leaves and cause the same symptoms as in fields.Consequently,this pathogen was identified as Cylindrocladium canadense.And this disease was named as the Cylindrocladium canadense leaf spot.
出处
《植物保护》
CAS
CSCD
北大核心
2012年第1期177-179,共3页
Plant Protection
基金
河南科技大学博士启动基金项目(200709001215)
河南省科技厅重点科技攻关项目(102102110090)
关键词
牡丹
叶斑病
柱枝孢
Paeonia suffruticosa
leaf spot
Cylindrocladium canadense
