摘要
利用不同浓度的苦楝树皮和叶、隆缘桉叶和乌桕叶的乙醇提取物对菟丝子及其宿主大豆幼苗进行处理,15d后评价三种提取物对菟丝子和大豆幼苗的影响。结果表明:三种提取物在低浓度时对菟丝子及其宿主大豆幼苗生长发育均无显著影响,在高浓度(0.25g/mL)下,桉树叶提取物对大豆和菟丝子的损伤程度分别达到了64%和70%,苦楝树皮提取物对菟丝子损伤程度为78%,但对大豆幼苗仅为7%。桉树叶和苦楝树皮提取物处理均导致大豆叶片超氧化物歧化酶(SOD)活性升高,最高值分别为相应对照组的2.37倍和2.0倍;但对过氧化物酶(POD)和过氧化氢酶(CAT)活性的影响不同,桉树叶提取物使大豆幼苗POD活性最大值为对照组的2.28倍,而苦楝树皮提取物处理则是CAT活性升高,最大值为对照组的1.58倍,提示桉树叶提取物对大豆较强的伤害作用与其较低的CAT活性有关。
The ethanol extracts of Melia azedarach＇s bark and leaves,Eucalyptus robusta＇s leaves,Sapium sebiferum＇s leaves were studied which treated to dodder and its host soybean seedlings in different concentrations,the impact of three plants extracts on dodder and soybean seedlings were evaluated at the 15 days after spraying.The results showed that three extracts in low concentrations had no significant influence on the growth and development of dodder and soybean seedling.In high concentrations （0.25 g/mL）,Eucalyptus robusta＇s leaves extract caused damage to soybean seedling and dodder reached at 64% and 70% respectively,while the damage of Melia azedarach＇s bark extract was 78% to dodder and only 7% to soybean seedlings.On the other hand,the treatment of Eucalyptus robusta＇s leaves and Melia azedarach＇s bark extract leaded to superoxide dismutase （SOD） activity of soybean＇s leaves rising,the highest values were 2.37 times and 2.0 times respectively as much as the control groups.But the effects in activities of peroxidase （POD） and catalase （CAT） were different,which the highest value of POD activity caused by Eucalyptus robusta＇s leaves extract was 2.28 times compared to the control group.Whereas CAT activity rose highest under Melia azedarach＇s bark extract treatment,the maximum was 1.58 times than the control group,which suggested the damage to soybean caused by Eucalyptus robusta＇s leaves extract was associated with its lower activity in CAT.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2012年第1期63-69,共7页
Genomics and Applied Biology
基金
广西大学国家大学生创新性实验计划资助项目(101059331)
广西大学科研基金资助项目(XB2100153)共同资助
关键词
植物提取物
菟丝子
苦楝
隆缘桉
乌桕
大豆
宿主植物
保护酶
Plant extract
Dodder （Cuscuta chinensis Lam.）
Melia azedarach
Eucalyptus robusta
Sapium sebiferum
Soybean （Glycine max）
Host plant
Protective enzymes
