摘要
以甘肃主要推广春小麦品种陇春22幼胚为转基因受体材料,建立了农杆菌介导的小麦遗传转化体系。以预培养4天的幼胚愈伤组织为受体,C58c1农杆菌菌株为供体,将含有半夏凝集素基因的重组质粒pBIpta转入了小麦,经G418 25 mg/L抗性筛选、PCR检测和荧光定量PCR检测共获得转基因植株3株,外源基因的插入拷贝数分别为2、1、3。同时对转基因小麦的T1代植株进行了PCR检测和抗虫性分析,表明半夏凝集素基因在转基因植株的后代中得到了遗传并有一定的抗蚜虫作用。
Agrobacterium tumefaciens-mediated wheat genetic transformation system was set up by using immature embryos of spring wheat cultivar Longchun 22 as explant. 4-day-preculture immature embryos were transformed with pta gene using Agrobacterium tumefaciens C58cl. Three transgenic plants were gained after G418 resistant screening and PCR analysis. Real-time PCR analysis showed that pta gene copy number in transgenic wheat plant is 2,1,3, respectively. The exogenous pta gene have integrated into three plants out of six T1 progeny plants confirmed by PCR analysis, and the transgenic wheat showed good resistance to aphids in greenhouse.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2012年第2期50-56,共7页
China Biotechnology
基金
甘肃省科技攻关项目(GS035-A41-001-03)
甘肃省农业生物技术应用与开发项目(GNSW-2004-06)资助项目
