摘要
为了研究生长分化因子11(growth differentiation factor,GDF11)基因与蒙古羊多脊椎性状间的关系,本研究首先克隆了该基因启动子区序列,并采用相关生物信息学软件对该序列进行了分析。结果得到512bp的蒙古羊GDF11基因启动子区序列,整个序列碱基构成为A占10.55%,G占16.80%,T占31.84%,C占40.82%,整个序列G+C含量百分比为57.62%。通过在线软件对蒙古羊GDF11基因启动子区生物信息学分析结果表明,该区域未找到符合条件的CpG岛,也未发现TATA box或CAAT box结构,但存在一处潜在的转录起始位点和HSF2、HSF2、GATA-1、AML-1a和MZF1 5个潜在转录因子,并且具有5种基序结构:EGF_1、CTCK_1、ANAPHYLATOXIN_1、VWFC_1和DEFENSIN。本研究结果为进一步揭示该基因对蒙古羊脊椎数的调控机理提供了重要的理论依据。
In order to analysis the relationship between growth differentiate factor 11（GDF11） and the traits of more vertebras of Mongolian sheep,the gene promoter region was cloned firstly and analysised by the bioinformatics software.The results showed that the GDF11 gene promoter region length was 512 bp and bases proportion were A 10.55%,G 16.80%,T 31.84%,C 40.82%,the proportion of G＋C was 57.62%.The results of bioinformatics analysis showed no CpG island and TATA box or CAAT box found in the Mongolian sheep GDF11 gene promoter region,but existed one transcription initiation site,5 transcription factors： HSF2,HSF2,GATA-1,AML-1a,MZF1,and 5 motifs： EGF_1,CTCK_1,ANAPHYLATOXIN_1,VWFC_1,DEFENSIN.The research provided theoretical basis for the Mongolian sheep vertebra regulation mechanism by GDF11 gene.
出处
《中国畜牧兽医》
CAS
北大核心
2012年第2期121-124,共4页
China Animal Husbandry & Veterinary Medicine
基金
内蒙古自治区农牧业科学院青年创新基金和国家肉羊产业技术体系(CARS-39)
