摘要
目的:模拟研究酸碱度和异烟肼对利福平服药后降解的作用。方法:将利福平、利福平与不同比例的异烟肼混合,放入盛有pH 6.8缓冲溶液和pH 1盐酸溶液的溶出杯中,37℃,100 r.min-1的条件下,在0,1,2 h取样,用HPLC法测定利福平和三甲酰利福霉素的含量,观察利福平的降解情况。结果:利福平在碱性环境(pH 6.8)中很少降解,在酸性条件下降解增加,加入异烟肼后降解显著增加。结论:利福平在酸性条件下,与异烟肼同时存在时降解明显增加。
Objective： To investigate the influence of pH on degradationof rifampicin combined with isoniazid after oral administration.Methods： Rifampcin and isoniazid were mixed in different proportions.The mixture was added into cups containing dissolution buffer solutions at pH 6.8 or hydrochlric acid solution at pH1,at 37 ℃,and agitated at 100 r·min-1.Under the conditions,samples were taken at 0,1 and 2 h.An HPLC method was adopted to determine rifampcin and 3-formyl rifamycin contents.Results： The degradation of rifampcin increased in the acidic condition,and rarely occurred in the alkaline condition.However,the degradation significantly increased after adding isoniazid.Conclusion： In acidic conditions and in the presence of isoniazid,the degradation of rifampicin can be increased.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2012年第2期202-205,共4页
Chinese Journal of New Drugs
基金
河南省重点技术创新项目(2002232)
关键词
利福平
异烟肼
高效液相色谱
降解
rifampicin
isoniazid
high performance liquid chromatography
degradation
