摘要
首次尝试利用电喷雾电离质谱(ESI MS)和氢原子核磁共振(1 H NMR)技术分析了含笑属六种植物种子的磷脂特性,发现在两个指纹图谱区发现明显的差异,即在质荷比(m/z)895-910(ESI MS)和5.30～5.40mg/L(1 H NMR)两个特异的区域存在显著差异。这些源于种子磷脂ESI/MS和1 H NMR的谱带差异可以被用来分析不同植物的种子的磷脂特征。而且,相似地,在更广的层面上,特异性的谱带差异可用于分析其他植物种子的磷脂组成和特性,辅助鉴定种子。
To date,there was no example to authenticate seed phospholipids based on their fingerprinting.By means of electrospray ionization mass spectrometry（ESI MS） and proton nuclear magnetic resonance（1H NMR） spectroscopy,phospholipids fraction extracted from seeds of six species from the genus Michelia were detected.It was firstly found that distinct difference in spectral fingerprinting region between m/z 895-910 in ESI MS and 5.30-5.40 mg/L in 1H NMR among these six species.Thus,it suggested that the spectral differences shown by ESI MS and 1H NMR among seeds of these six species could be applied to identify them.And in a wider sense,for analyzing seed phospholipids of other plant species,a combination of ESI MS AND NMR was an effective tool.
出处
《广西植物》
CAS
CSCD
北大核心
2012年第1期129-133,共5页
Guihaia
基金
Supported by the Particular Biological Conservation Foundation of the Chinese Academy of Sciences(KSCX2-YW-Z-0925)
Chongqing Education Foundation(KJ100610)~~
关键词
种子磷脂
质谱
核磁共振
指纹
含笑属
Seed phospholipid
ESI MS
NMR
Fingerprinting
Michelia
