摘要
目的:探讨控制性肺膨胀(SI)对Beagle犬急性呼吸窘迫综合征(ARDS)开放吸痰后炎症相关细胞因子的影响。方法:选择12只Beagle犬,利用油酸建立犬ARDS模型,稳定30min后随机进行两组吸痰,SI组吸痰后立即予以一次SI。非SI组吸痰后不予SI。利用ELISA方法检测非SI组及SI组在使用油酸成模前、成模稳定后、吸痰后1h和4h时间点血清中肿瘤坏死因子α(TNF-α)(pg/ml)、白介素1β(IL-1β)(pg/ml)、白介素6(IL-6)(pg/ml)、白介素10(IL-10)(pg/ml)的水平。结果:Beagle犬经油酸成模后,各类炎症相关细胞因子特别是炎性细胞因子TNF-α、IL-1β、IL-6,及抑炎细胞因子IL-10水平较使用油酸成模前均显著升高(P<0.01);与此同时,与单纯吸痰(非SI组)相比而经吸痰和控制性肺膨胀(SI组)后,上述细胞因子均有下降(P<0.05),并且这种作用随着吸痰后通气时间的延长而延长。结论:SI能改善ARDS开放吸痰后肺的氧合指标,改善肺顺应性的分子基础可能是改善组织(特别是心肺组织)炎症程度。
Objective： To evaluate the treatment value of sustains inflation （SI） in inflammation-related cytokines of serum after open endotrachel suctioning in acute respiratory distress syndrome （ARDS）. Methods： Chose twelve Beagle canines, oleic acid-induced ARDS canine models were randomly divided into two groups of open endotrachel suctioning after stabilizing for 30 min. SI-group： used SI in ARDS Beagle dog after open endotrachel suctioning. Non-SI-group： did not use SI in ARDS Beagle dog after open endotrachel suctioning. Inflammation-related cytokines of serum such as TNF-α, IL-1β, IL-6 and IL-10 were measured at 4 time points （before OA, OA exposure, open endotrachel suctioning 1 h, open endotrachel suctioning 4 h ） by ELISA assay. Results： All inflammation-related cytokines were increased after open endotrachel suctioning at the 3 time points in the two groups, including the anti-inflammation cytokine TNF-α, IL-1β, IL-6, IL-10 （P0.01）. However, there were less augment in SI-group inflammation-related cytokines compared with Non-SI-group （P0.05）. Furthermore, the less augment was time-dependent decrease. Conclusion： The molecular mechanism of effect of SI in better oxygenation and lung compliance is attributed to improve the degree of inflammation.
出处
《中国当代医药》
2012年第1期8-9,共2页
China Modern Medicine
关键词
急性呼吸窘迫综合征
控制性肺膨胀
开放吸痰
细胞因子
全身性炎症反应综合征
Acute respiratory distress syndrome （ARDS）
Sustains inflation（SI）
Open endotrachel suctioning
Inflammation-related cytokines
Systemic inflammatory response syndrome （SIRS）
