摘要
本研究,我们以6个花生品种为亲本配置杂交组合,田间调查发现亲本间形态表型差异大的杂交F1容易鉴别真伪,而形态表型差异小的则难于鉴别,甚至无法鉴别。为此,我们通过改良Thomson一步法制备DNA模板,建立了一套花生SSR-PCR快速检测体系,并在此基础上利用SSR鉴别花生杂交F1真伪。结果表明,采用Thomson一步法和改良后的一步法提取的DNA模版均能扩展出清晰条带,但改良后制备的DNA模板在4℃下可保存约1个月,未改良的仅能保存一天。利用SSR检测上述群体表明,F1真杂种率为38%～56%,不同亲本间杂交成功率存在差异,同一亲本正反交成功率也存在一定差异。可见,SSR标记用于杂种真伪鉴定具有一定的应用潜力。
In this research,we used six peanuts varieties as parents to make hybrid combinations for hybrid identification.Our practice showed that the authenticity of F1 hybrids would be easy validated by field agronomic investigation,if whose parents had visible and distinguishable phenotypic differences,otherwise it might be very difficult to identify the authenticity of F1 hybrids.Therefore,we established an SSR-PCR rapid detection system for peanut identification by modifying Thomson＇s one-step protocol to prepare DNA template,which was applied to identify the authenticity of peanut F1 hybrids.The results showed that DNA templates extracted from Thomson＇s one-step protocol and modified Thomson＇s one-step protocol were able to generate sharp bands.Peanut DNA prepared by the modified protocol stored at 4℃ for almost a month was still fresh and working well,whereas peanut DNA prepared by Thomson＇s was completely degraded only staying a day at 4℃.The authenticity of F1 hybrid combinations in this study were identified to be 38%~56%,the results further indicated that hybrid rates should be differences among different parents,even one pair parents for reciprocal cross.Therefore,we thought that using SSR markers to identify the authenticity of peanut hybrid might be great potential in future.
出处
《分子植物育种》
CAS
CSCD
北大核心
2012年第1期110-114,共5页
Molecular Plant Breeding
基金
国家自然科学基金项目(30900907,30971819)
广东省自然科学基金项目(9151007010000001)
粤港关键领域重点突破项目(2008A024200009)共同资助
