摘要
【目的】探讨不同免疫途径沙眼衣原体(Chlamydia trachomatis,Ct)分泌性蛋白Pgp3的免疫保护效果,分析其可能的保护机制,以确定Pgp3蛋白疫苗在Ct疫苗研制中的应用价值。【方法】分泌性蛋白Pgp3经滴鼻或肌注途径免疫雌性Balb/c小鼠,免疫60 d后,阴道接种鼠沙眼衣原体(Chlamydia muridarum,Cm)建立生殖道感染动物模型,在该模型中评价Pgp3蛋白疫苗抗Cm感染的保护效果,并探讨其机制。【结果】滴鼻或肌注免疫后,小鼠血清及生殖道中检测到了特异性抗体;小鼠脾淋巴细胞产生IFN-γ、IL-17及IL-5水平均明显高于对照组,且滴鼻免疫组IFN-γ水平升高较肌注组更显著(P<0.05);Pgp3蛋白滴鼻免疫组小鼠经Cm生殖道感染后,阴道带菌时间明显缩短,输卵管病理改变轻而肌注免疫组其保护作用不明显。【结论】Pgp3蛋白经滴鼻免疫可有效诱导小鼠产生明显的抗Cm保护效应。其可能的免疫保护机制与诱导Th1型为主的细胞免疫应答及高效价的特异性抗体有关,提示Pgp3蛋白疫苗具有潜在的疫苗研究与开发价值。
[Objective] To validate the immune protective efficacy of Chlamydia secretion protein Pgp3 and to analyze the potential immune mechanisms related to this protection.[Methods] The prokaryotic recombinant protein Pgp3 was purified and evaluated for its protective efficacy in a genital tract infected mouse model.Groups of BALB/c mice were immunized intranasally or intramuscularly with adjuvants plus Pgp3 protein or PBS and GST control.Humoral and cellular immune responses were evaluated.After Chlamydia muridarum intravaginal challenge,the chlamydia shedding from the lower genital tract and the chlamydia-induced upper genital tract gross pathology and histopathological characterization were also detected.[Results] Adjuvant plus Pgp3 immunization can induce high level of Pgp3 specific antibody as well as IFN-γ,IL-17 and IL-5 cytokine production.More importantly,intranasal immunization compare with intramuscular route induced more Th1-dominant immunity that significantly reduced the shedding of live organisms from the lower genital tract and attenuated inflammatory pathologies in the oviduct tissues.[Conculsion] These observations have demonstrated that secretion protein Pgp3 intranasal immunization can induce protective immunity against chlamydial infection and pathology in mice.
出处
《微生物学通报》
CAS
CSCD
北大核心
2012年第2期226-236,共11页
Microbiology China
基金
国家自然科学基金项目(No.30970165)
湖南省高校科技创新团队(湘教通[2010]53号)
