摘要
目的了解热应激对小鼠精原细胞增殖情况的影响,探讨高温造成男性短期不育的机制。方法 8周龄雄性昆明白小鼠于43℃水浴中进行睾丸局部热应激处理15min,于一次性处理后1、12、18、25d以及35d通过PCNA免疫荧光组化法检测精原细胞增殖情况;于一次性处理后4、2、10、15、20d以及25d通过RT-PCR检测GDNF和SCF基因表达情况。结果在热处理后第12d和18d,精原细胞增殖系数分别为22%和75%,明显低于(P<0.05)对照组的93%;25d时,精原细胞增殖水平恢复至正常水平。GDNF mRNA的表达量在处理后4h到第15d显著下调(P<0.05);SCF mRNA的表达量在热处理后第4h到第10天显著下调(P<0.05)。结论睾丸局部热应激处理使得GDNF和SCF基因表达在处理后短时间内下调,精原细胞增殖短时间内抑制。
Objective To understand the effect of heat shock on mouse spermatogonia proliferation,and to explore the mechanism of male short-term infertility caused by high-temperature.Methods Eight-week-old male KM mice were treated with 43℃ heat shock for 15 minutes.Spermatogonia proliferation and GDNF and SCF gene expression was detected by PCNA immunofluorescence staining at the 1,12,18,25d and 35d after heat shock-treatment and by RT-PCR at the 4h,2d,10d,15d,20d and 25d after heat shock-treatment,respectively.Results At the 12th and 18th day after heat-shock treatment,spermatogonia proliferation index were 22% and 75%,which were significantly lower（P〈0.05） than 93% in the control group.At the 25th day after heat-shock treatment,the proliferation levels returned to normal levels.The expression levels of stem cell proliferation promoting regulatory factor GDNF in spermatogonial stem-cell micro-environment significantly（P〈0.05） down-regulated during the 4thh to 15th day after shock treatment;Stem cell differentiation regulatory factor SCF expression levels was significantly（P〈0.05） down-regulated at the 4thh to 10th days after heat shock treatment.Conclusion The expression of GDNF and SCF genes significantly reduced shortly after heat shock treatment,and permatogonia proliferation was inhibited.
出处
《宁夏医科大学学报》
2011年第12期1131-1134,F0003,共5页
Journal of Ningxia Medical University
基金
宁夏自然科学基金项目(NZ1080)
