摘要
目的 探讨不同应力刺激下犬股骨头缺血性坏死后自身修复过程中血管内皮生长因子（VEGF）蛋白及mRNA的表达变化及意义.方法 健康比格犬24只,通过液氮冷冻法制成犬股骨头坏死,并对坏死股骨头施以不同的应力,根据承受应力环境不同分为低高及中应力组（B、C、D组）.对照组（A组）股骨头不做处理.分别于术后4、8周处死实验犬,取股骨头标本,行HE染色观察组织病理学改变,免疫组化染色及定量逆转录聚合酶链式反应（RT-PCR）检测VEGF蛋白及mRNA的表达变化.结果 HE染色提示：D组关节软骨、骨小梁的结构和形态较B、C组改善；免疫组化提示：D组股骨头内VEGF蛋白面积积光度在术后第4、8周显著高于其他各组；RT-PCR检测最示：D组股骨头VEGF mRNA的表达率在术后第4、8周高于其他各组,第8周高于第4周（均P＜0.05）.结论适当的应力刺激能有效促进犬股骨头骨组织中VEGF蛋白及mRNA的表达,进而有助于促进血管的新生和软骨下骨的重建,可能有利于坏死股骨头的修复.
Objective To explore the changes and its significance of the expression of vascular endothelial growth factor （VEGF） protein and messenger RNA in the Beagle dogs femoral head during the repairing process of the osteonecrosis of femoral head （ ONFH ） under different stress force stimulations.Methods Twenty-four Beagle dogs were selected to establish the model of ONFH through the method of liquid nitrogen cryopreservation.In the mean time,the necrotic femoral heads stimulated by different stress forces were enrolled into 3 groups： group B （low stress force stimulation group）,group C （high stress force stimulation group） and group D （average stress force stimulation group）.Group A was control group.The beagle dogs were sacrificed at Weeks 4 and 8 post-operation.The changes of femoral head were evaluated by histological observation,immunohistochemical staining and quantitative reverse transcription-polymerase chain reaction （RT-PCR）.Results Histological observation showed that articular cartilage,morphology and structure of trabecular bone in group D was better than those of groups B and C.Immunohistochemistry revealed that the VEGF protein positive staining in group D was significantly higher than that in groups A,B and C at Weeks 4 and 8 post-operation.The expression of VEGF mRNA in group D was much more than that in groups A,B and C by RT-PCR.And the expression was higher at Week 8 post-operation.Conclusion Proper stress force stimulation can effectively promote the expression of VEGF protein and messenger RNA in necrotic femoral head of Beagle dogs and boost angiogenesis to improve the blood supply of necrotic femoral head so as to accelerate the repairing process of ONFH.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2012年第1期40-44,共5页
National Medical Journal of China
基金
基金项目：国家自然科学基金（30970699）
关键词
股骨头坏死
局部缺血
物理刺激
内皮生长因子
Femor head necrosis
Ischemia
Physical stimulation
Endothelial growth factors
