摘要
采用噻唑蓝(MTT)法、碱性磷酸酶(ALP)比活性测定、油红O染色、Ⅰ型胶原测定以及矿化结节染色及定量分析等方法,研究了不同浓度的硝酸锶对原代培养的成骨细胞增殖、分化、矿化功能以及横向分化为脂肪细胞的影响。结果表明:硝酸锶对成骨细胞增殖、分化、矿化功能以及横向分化为脂肪细胞的影响与作用浓度和时间密切相关,但没有呈现出剂量依赖性。结果提示,硝酸锶对骨代谢的影响是复杂的,其具有保护还是损害作用取决于作用浓度和时间,而且它们是影响硝酸锶生物效应(从损伤到保护)转变的关键因素。
The effect of Sr（NO3）2 concentration on proliferation,differentiation and mieralizerzation function of primary osteblasts（OBs） in vitro was evaluated by using the 3-（4,5-dimethyl）-2,5-diphenyl tetra-zolium bromide（MTT）method,alkaline phosphatase（ALP） activity,oil red assays,quantitative analysis of promotion rate for type Ⅰ collagen production and Alizarin Red Stain（ARS）.The results indicate that the effect of Sr（NO3）2 on the proliferation,differentiation,mieralizerzation function and adipocytic transdifferentiation of OBs depends on the concentration and incubation time,however,the effect is independent of dosage.This suggests the effect of Sr（NO3）2 on bone metabolism is a complex issue.Whether the effect of Sr（NO3）2 is damaging or protection is dependent on two key factors,i.e.,the concentration and culture time.
出处
《无机化学学报》
SCIE
CAS
CSCD
北大核心
2012年第2期374-380,共7页
Chinese Journal of Inorganic Chemistry
基金
国家自然科学基金(No.20971034)
河北省自然科学基金重点(No.B2009000161)资助项目
关键词
硝酸锶
成骨细胞
增殖
分化
矿化
strontium nitrate
osteoblasts
proliferation
differentiation
mineralization
