摘要
目的探讨索拉非尼对人卵巢癌SKOV-3细胞凋亡的影响。方法采用MTT法,检测不同浓度索拉非尼(1.5、3.0、6.0、12.0、24.0μmol/L)作用人卵巢癌SKOV-3细胞24、48、72 h的抑制率;Hoechst染色法观察药物处理后细胞凋亡情况;应用索拉非尼与紫杉醇不同给药方式作用于SKOV-3细胞,流式细胞仪检测药物处理后细胞周期和凋亡率变化。结果索拉非尼对SKOV-3细胞增殖具有明显抑制作用,与对照组比较差异有统计学意义(P<0.05),并且呈剂量和时间依赖性;索拉非尼主要使SKOV-3细胞周期阻滞在S期,与紫杉醇联合给药后S期及G2/M期均有延长,且凋亡率较高(P<0.05)。结论索拉非尼对SKOV-3细胞增殖有显著抑制作用,且呈剂量和时间依赖性,紫杉醇序于索拉非尼给药时细胞凋亡率更高。
Objective This study was to evaluate the effect of sorafenib on human ovarian cancer cell SKOV-3.Methods MTT was used to examine the effects of sorafenib at different concentrations（1.5、3.0、6.0、12.0 and 24.0 μmol/L） and different time points（24、48 and 72 h） on proliferation of SKOV-3 cells.Hoechst 33258 staining assay was used to observe sorafenib-induced morphological changes.SKOV-3 cells were treated with sorafenib or paclitaxel alone or in three different schedules：sorafenib was given prior to,after or simultaneously with paclitaxel.Alteration of cell cycle and apoptosis were analyzed by flow cytometry.Results The results indicated that sorafenib could induce apoptosis of SKOV-3 cells and the apoptotic rate was increased with the increase of sorafenib concentration compared with the control group（P0.05）.Sorafenib caused cell cycle arrest at S phase,S and G2/M phases were extended when sorafenib was in combination with paclitaxel.Conclusion Sorafenib can effectively inhibit the proliferation of SKOV-3 cells in time and dose dependent manners.Administration of sorafenib after paclitaxel induces a higher apoptotic rate in SKOV-3 cells than administration before or simutanously with paclitaxel.
出处
《实用癌症杂志》
2012年第1期12-14,17,共4页
The Practical Journal of Cancer
关键词
索拉非尼
凋亡
卵巢癌
Sorafenib
Apoptosis
Ovarian cancer
