摘要
目的:建立用薄层色谱法(TLC)同时定性分析和反相高效液相色谱法(RP-HPLC)同时定量分析黄金菊片剂中药效组分黄芩苷-绿原酸-荭草苷-牡荆苷的方法,为建立中药与临床疗效对应的药效组分质量评价体系奠定基础。方法:TLC鉴别是以聚酰胺薄膜为固定相,以氯仿-乙酸乙酯-甲醇-甲酸(5:5:4:0.6)为展开剂,喷以1%三氯化铝乙醇溶液,置紫外光灯365nm下检视。RP-HPLC法以Agela Venusil为色谱柱,以0.2%磷酸水(A)-乙腈(B)(0-30min,B 15%-30%线性洗脱)为流动相,流速1mL/min,检测波长为340nm,并对专属性、线性、准确度和精密度等进行了方法学验证。结果:4种成分TLC法检测分离度较好,RP-HPLC法标准曲线线性关系良好,R2均大于0.9995,平均回收率为98.99%-100.33%,相对标准偏差RSD均小于1.79%。结论:两种方法均操作简便、结果准确、灵敏度高,可用于黄金菊片剂的质量控制。
Objective： To establish thin-layer chromatography（TLC） and reversed-phase high-performance liquid chromatography（RP-HPLC） methods for simultaneous qualitative and quantitative analysis of the active components alignment（ACA）（baicalin-chlorogenic acid-orientin-vitexin） in Huangjinju Tablets.To lay a foundation for the establishment of the active components alignment quality evaluation system of traditional Chinese medicine based on clinic curative effects.Methods： TLC chromatogram of the active components alignment was obtained on a polyamide sheet with a system consisting of chloroformethyl acetate-methanol-formic acid,5 ：5：4：0.6（v/v/v/v）.Visualization was accomplished with UV light（365nm） after spraying the sheets with 1% aluminium chloride in ethanol.RP-HPLC method involves a gradient elution on an Agela Venusil column using solvent A,0.2% phosphoric acid in water and solvent B,acetonitrile as mobile phase at a flow rate of 1mL/min.A linear gradient from 15% to 30% of solvent B in 30min.UV detection was monitored at 340nm.The HPLC method was validated for specificity,linearity,accuracy and precision.Results： Four compounds in Huangjinju Tablets were successfully separated from each other.The HPLC standards showed a good linearity for the four analytes with determination coefficients higher than 0.9995.The average recoveries were ranged from 98.99% to 100.33% with RSD less than 1.79% for these components.Conclusion： Both methods were proved to be simple,accurate and sensitive and are applicable to the quality control of Huangjinju Tablets.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2012年第2期303-308,共6页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
广东省教育部产学研结合项目(No.2007B090400085)~~
