摘要
目的评价傅里叶频域光学相干断层成像技术(FD-OCT)在大鼠急慢性视神经损伤动物模型中的应用及其与组织学研究的一致性。方法利用经眶部视神经切断方法及巩膜上静脉烧灼方法建立大鼠急慢性视神经损伤动物模型。在急性视神经损伤模型中,于视神经切断术后即刻、3d、7d、10d、14d,分别用FD-OCT、视网膜神经节细胞(RGC)逆行标记、视神经纤维顺行标记方法检测视神经损伤状态。在慢性视神经损伤模型中,于巩膜上静脉烧灼术后即刻、1周、2周、3周、4周、5周、6周分别行上述方法检测。所有实验动物右眼为实验眼,左眼为对照眼。统计数据采用SPSS13.0软件进行分析,不同时间点的视网膜厚度比较采用重复测量资料的方差分析。结果在急性视神经损伤动物模型中,视网膜厚度由术前(71.6±0.7)μm逐渐在术后3d、7d、10d、14d分别减薄至(71.0±0.9)μm、(67.4±1.7)μm、(58.6±2.2)μm、(54.6±1.2)μm,RGC数量在各时间点减少为对照组的(90.6±1.3)%、(53.1±3.8)%、(30.2±1.0)%、(11.3±4.6)%。在慢性视神经损伤动物模型中,视网膜厚度由术前(71.4±1.3)μm逐渐在术后1周、2周、3周、4周、5周、6周分别减薄至(70.4±1.1)μm、(65.9±1.2)μm、(59.9±1.0)μm、(57.3±2.4)μm、(54.7±2.9)μm、(51.3±2.6)μm,RGC数量在各时间点减少为对照组的(95.9±0.7)%、(91.9±0.9)%、(85.5±1.3)%、(82.3±0.9)%、(78.1±1.5)%、(74.5±1.4)%。两种动物模型中,视网膜厚度变薄及RGC数量减少与对照组相比差异均具有统计学意义。结论 FD-OCT对于评价慢性视神经损伤动物模型具有良好的应用价值,与组织学检测具有良好的一致性。
Objective To evaluate the integrity of Fourier domain-optical coherence tomography （FD-OCT） and histologic study in rat acute and chronic retina neurodegeneration animal model. Methods Optic nerve transection and episcleral vein cauteriation （EVC） animal models were used in this study. In acute axotomy animal model, FD-OCT,number of retinal ganglion cells （ RGCs）, and optic nerves fiber were studied immediately and at day 3, 7, 10, 14 after the surgery respectively. In chronic EVC animal model, the measurements were assesed immediatly and in week 1,2, 3, g, 5, 6 after the surgery respectively. In all of the experiments, the right eye was considered to be the experimental eye, while the contralateral eye was control. Statistics were performed by SPSS13.0 software. Results In axotomy rat model, retinal thickness reduced from （71.6 ± 0.7） μm before surgery to （71.0 ± 0. 9） μm, （67.4 ± 1.7 ） μm, （58.6± 2.2）μm and （54.6 ± 1.2）μm at day 3, 7, 10, 14 after the surgery respectively, while RGCs were reduced to （90.6±1.3） %, （53.1±3.8） %, （30.2± 1.0） %, （ 11.3 ± 4.6） % respectively. In EVC model, retinal thickness reduced from （71.4 ± 1.3 ）μm before surgery to （70.4 ± 1.1 ） txm, （65.9 ± 1.2） μm, （59.9 ± 1.0） μm, （ 57.3 ± 2.4）μm, （54.7 ±2.9）μm, （51.3 ±2.6）μm in week 1, 2, 3, 4, 5, 6 after the surgery respectively, while RGCs reduced to （95.9 ±0.7）%, （91.9±0.9）%, （85.5±1.3）%, （82.3 ±0.9）%, （78.1 ±1.5）%, （74.5±1.4）% respective ly. In both animal models, both the reduction of retina thickness and the RGCs number were statistically different from those in the contralateral eyes. Conclusions FD-OCT had favorable utilization value in the evaluation of neurodegenera- tion in chronic retina injury animal models, which was in consistent with histologic study. （ Chin J Ophthalmol and Otorhi- nolaryngo1,2012 ,12 ：20-25 ）
出处
《中国眼耳鼻喉科杂志》
2012年第1期20-25,共6页
Chinese Journal of Ophthalmology and Otorhinolaryngology
基金
国家自然科学基金(30872832)
广东省高等学校高层次人才项目(2010)
眼科学国家重点实验室创新基金(2010)
广东省自然科学基金重点项目(10251008901000028)
