摘要
目的：研究生理脉动切应力对血管内皮细胞一氧化氮产生的影响。材料与方法：利用本室已建立的平行板模拟血流循环装置，在模拟生理脉动切应力（１９． ２９，５６． ２５ ｄｙｎｅｓ／ｃｍ２）分别作用于培养人脐静脉内皮细胞 ０，１，２，４，６，１０，２４ ｈ后，用一氧化氮微量测定法测定循环液中一氧化氮的含量。结果：在两组模拟生理脉动切应力的作用下，于２ｈ时一氧化氮释放出现一峰值，之后逐渐下降，并且内皮细胞释放一氧化氮的量随切应力的增大而增加。结论：生理脉动切应力对血管内皮细胞一氧化氮的产生具有调节作用，可能对血管动脉粥样硬化的形成与发展中，临床手术前后发生的血管痉挛与血栓形成等增多有重要影响。
To study the effect of physiologic pulsatile shear stress of low on nitric oxide synthesis by cultured endothelial cells (EC). Methods: The parallel plate flow-loading apparatus was used to imitate the physilogic pulsatile shear stress (19. 29 and 56. 25 dynes/cm2) and the concentrations of nitric oxide (NO) in flow were measured among test groups and different time points. Results: NO production by EC were increased under the pulsatile shear stress condition, the releasing of NO reached its peak on 2 h, and then it declined gradually. The production of NO by EC increased with the increase of the pulsatile shear stress. Conclusion: This results indicate that physiologic pulsatile shear stress modulate the production of NO by EC.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2000年第1期11-14,共4页
Chinese Journal of Anatomy
基金
卫生部科研基金资助项目!(No. 94-1-149)
关键词
内皮细胞
一氧化氮
生理脉动切应力
endothelial cell
physiologic pulsatile shear stress
nitric oxide
