摘要
首次在长牡蛎(Crassostrea gigas)中克隆得到细胞周期蛋白B3(cyclin B3)的cDNA全长序列和基因组结构序列。cyclin B3基因cDNA全长2383 bp,其中编码区长度为1293 bp,编码一条含430个氨基酸的多肽链,氨基酸序列比对和结构域分析均表明其为其他物种cyclin B3的同源蛋白,预测的蛋白大小为47.8 kD。cyclin B3基因含有10个外显子和9个内含子,外显子和内含子的数目和大小与其物种的进化地位相符。Real Time PCR分析表明,该基因表达有较强的组织特异性,cyclin B3 mRNA在性腺中的含量最高,而在外套膜中的含量最少,前者是后者的92倍左右;对处于不同发育阶段性腺中cyclin B3基因表达分析结果显示,性腺中cyclin B3 mRNA含量随性腺发育程度的提高而提高,这与其在细胞分裂和减数分裂中的作用相符,同时也有助于满足早期胚胎发育时期旺盛的细胞分裂对cyclinB3蛋白需求。
The full length cDNA sequence of cyclin B3 was cloned from Pacific oyster （Crassostrea gigas） for the first time. Cyclin B3 cDNA was 2383 bp in length, containing a 1293bp CDS that encoded a peptide of 430 amino acids with a calculated molecular weight of 47.8 kD. Multiple alignment and converted domain analysis showed that this peptide was a homolog of cyclin B3. The gDNA sequence of cyclin B3 contained 10 exons and 9 introns, which was consistent with the position in evolution of C. gigas. Expression of cyclin B3 gene was found in all tissues, but also showed a very strong tissue-specific feature; the amount of cyclin B3 in gonad was the highest among tissues, 100 times of mantle, the lowest expression tissue. The mRNA amount of cyclin B3 in gonad increased with the maturation of gonad. The expression implied the important role of cyclin B3 in mitosis, meiosis and early development of embryo.
出处
《海洋科学》
CAS
CSCD
北大核心
2011年第12期1-9,共9页
Marine Sciences
基金
国家"863"计划资助项目(2006AA10A401)
国家"973"计划资助项目(2010CB126401)
国家公益性行业(农业)科研专项(3-53)
