摘要
目的探讨乙肝大三阳携带者精浆乙肝病毒核酸（HBVDNA）的检测方法、含量及其影响因素。方法选择男性乙肝大三阳携带者99例，根据血清HBVDNA定量级别分为10，组和10^8组，采用沉淀煮沸裂解法（实验组）和微量DNA提取法（DNAMini法）（对照组）抽提精浆的HBVDNA模板，进行荧光定量PCR检测。结果实验组和对照组检测精浆HBVDNA的阳性率分别为62．0％和61．2％，两者比较差异无统计学意义（P〉0．05）。经2种DNA抽提法检测的10，组和108组精浆HBVDNA的阳性率分别为35．3％和89．6％，差异有统计学意义（P〈0．01）。经对数转换后，血清与精浆HBVDNA含量分别为（7．98±0．42）IU／ml和（3．05±1．58）IU／ml，差异有统计学意义（P〈0．01）。结论沉淀煮沸裂解法能有效抽提精浆的HBVDNA进行荧光定量PCR测定，乙肝大三阳携带者精浆HBVDNA含量低于血清HBVDNA含量，精浆HBVDNA的阳性率与血清HBVDNA水平密切相关。
Objective To investigate the hepatitis B virus （HBV） DNA testing method, its level and influential factors in seminal plasma of male carriers with HBsAg（＋）, HBeAg（＋） and HBc（＋）. Methods Ninety-nine hepatitis B male carriers were divided intol07 and 108 group according to the log 10 HBV DNA in serum. The precipitating and boiling extraction method （experimental group） and QIAamp DNA Mini method （control group） were used to extract nucleic acid of HBV and HBV DNA was detected with fluorescent quantitative analysis. Results The positive rate of HBV DNA in seminal plasma in the experimental and control groups was 62.0% and 61.2% respectively. The results of the two groups were similar （P〉0.05）. The positive rate of HBV DNA in seminal plasma in the 10s group （89.6%） was higher than that in the 107 group（35.3%）（P〈0.01）. The amount of HBV DNA in seminal plasma was lower than that in serum （P〈0.01）. Conclusion The precipitating and boiling extraction method is efficient for extraction ofHBV DNA in seminal plasma and suitable for HBV DNA quantitative PCR detection. The amount of HBV DNA in seminal plasma is low and its positive rate is closely related to the amount of HBV DNA in serum.
出处
《中国男科学杂志》
CAS
CSCD
2011年第12期30-33,共4页
Chinese Journal of Andrology
基金
本课题受温州市科技局科研基金资助（Y20090358）
关键词
肝炎病毒
乙型
聚合酶链反应
精液
hepatitis B virus
polymerase chain reaction
semen
