摘要
目的:监测小鼠心肌缺血再灌注损伤(MIRI)过程中血清炎性细胞因子IL-6和TNF-α水平变化,以及瘦素预处理对其的影响,探讨瘦素预处理在小鼠MIRI中的作用。方法:40只雄性C57BL/6小鼠随机分为假手术组、MIRI模型组、MIRI瘦素预处理组(小鼠在MIRI手术前30min给予腹腔注射重组瘦素)和MIRI术后瘦素处理组(小鼠于MIRI手术后30min给予腹腔注射重组瘦素),在MIRI手术后3和6h分别取小鼠血清及心肌组织,测定小鼠血清炎性细胞因子IL-6和TNF-α水平以及心肌、血清瘦素水平。结果:与假手术组比较,MIRI瘦素预处理组3和6hIL-6和TNF-α水平明显升高(P<0.01);与MIRI模型组比较,MIRI瘦素预处理组3和6hIL-6和TNF-α水平明显降低(P<0.01)。与假手术组比较,MIRI瘦素预处理组3和6h心肌、血清瘦素水平明显升高(P<0.05);与MIRI模型组比较,MIRI瘦素预处理组3和6h心肌、血清瘦素水平明显降低(P<0.01)。结论:瘦素预处理可减轻小鼠缺血心肌中炎性细胞因子IL-6和TNF-α的表达,从而起到心肌保护的作用;瘦素预处理可减轻MIRI。
Objective To monitor the changes of serum interleukin-6（IL-6） and tumor necrosis factor-alpha（TNF-α） levels in the process of myocardial ischemia reperfusion injury（MIRI） in mice and the pretreatment effects of leptin on them,and to explore the effects of leptin pretreatment on MIRI in mice.Methods Forty males C57BL/6 mice were randomly divided into sham-operation group,MIRI model group,established by blocking left coronary artery anterior descending branch for 30 min followed by reperfusion）,leptin pretreatment group（Leptin＋MIRI group,established by injecting intraperitoneally leptin at 30 min before ischemia）and leptin post-treatment group（MIRI＋Leptin group,established by injecting intraperitoneally leptin at 30 min after reperfusion）.The blood and heart samples were obtained from each mouse at 3 and 6 h after reperfusion.The serum IL-6 and TNF-α levels and the levels of leptin in cardiac muscle and serum were detected.Results Compared with sham-operation group,the serum IL-6 and TNF-α levels at 3 and 6 h in Leptin＋MIRI group were significantly increased（P0.01）.Compared with MIRI model group,the srum IL-6 and TNF-α levels at 3 and 6 h in Leptin＋MIRI group were signicantly decreased（P0.01）.Compared with sham-operation group,the leptin levels in cardiac muscle and serum at 3 and 6 h in Leptin＋MIRI group were significantly increased（P0.05）;compared with MIRI model group,the leptin levels in cardiac muscle and serum at 3 and 6 h in Leptia＋MIRI group were significantly decreased（P0.01）.Conclusion Leptin pretreatment can reduce the serum IL-6 and TNF-α expressions,thus may have cardiac muscle protection,and leptin pretreatment can alleviate MIRI.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2012年第1期58-61,共4页
Journal of Jilin University：Medicine Edition
基金
2011广西省科技厅广西科学研究与技术开发计划项目资助课题(桂科攻1140003B-76)
2010广西医疗卫生重点科研基金资助课题(重2010050)
2011广西研究生教育创新计划项目资助课题(桂学位[2011]35号-558)
