摘要
目的观察铁过载小鼠骨质疏松模型中血清骨转换指标的变化。方法腹腔注射生理盐水和枸橼酸铁铵建立对照和高铁小鼠模型。小动物活体成像系统测定小鼠双侧股骨中段和第四腰椎骨密度。酶联免疫吸附试验（ELISA）检测血清中铁蛋白、骨特异性碱性磷酸酶（BALP）、骨钙素（BGP）、抗酒石酸酸性磷酸酶（TRAP一5b）、I型胶原C端肽（CTX）、核因子KB受体活化因子配体（RANKL）、骨保护素（OPG）含量。结果高铁组小鼠双侧股骨中段和第四腰椎骨密度显著低于对照组（P〈0．05）。高铁组小鼠血清中铁蛋白、TRAP-5b、CTX、RANKL含量分别为（269．72±54．06）μg/L、（41．46±8．00）U／L、（7720．00±3554．00）pmol／L、（94．22±29．79）ng／L，显著高于对照组（101．18±17．10）μg/L、（19．57±9．02）U／L、（3140．00±632．00）pmol／L、（47．70±15．08）ng／L（P〈0．05）；BALP、BGP含量分别为（7．84±1．74）μg/L、（58．54±4．60）μg／L，明显低于对照组（18．63±6．23）μg/L、（100．99±18．22）μg/L（P〈0．05）；OPG含量两组之间差异无统计学意义（P〉0．05）。结论铁过载可能通过抑制骨形成，促进骨吸收引起小鼠骨质疏松。
Objective To explore the effect of iron overload on the bone turnover markers of mice serum. Methods The control and iron overload groups were established by the intraperitoneal injection of normal saline and FAC. In vivo imaging system was employed to determine the bone density of midportion of femur and the fourth lumbar vertebra before the treatment and at the end of first mouth and the second month of treatment. The ferritin, bone-specific alkaline phosphatase （BALP） , osteocalein （BGP） , tar- trate-resistant acid phosphatase 5b （TRAP-Sb）, type I collagen C-terminal peptide （CTX）, receptor acti- vator of nuclear factor Kb ligand （RANKL） and bone protection element （OPG） in serum were measured by using enzyme linked immunosorbent assay （ELISA）. Results The bone mineral density （BMD） of midportion of femur and the fourth lumbar vertebra in iron overload group was significantly lower than that in control group （ P 〈 O. 05 ）. The ALP and BGP in serum of iron overload group were （7.84 _＋ 1.74 ） and （58.54 ---4. 60） ixg/L, which were dramatically lower than those of control group [ （ 18.63±6.23 ） and （ 100. 99 ±18.22） I.Lg/L, P 〈 0. 05 ], while the concentrations of ferritin, TRAP-Sb, CTX and RANKL in iron overload group were （269.72±54.06） txg/L, （41.46±8.00） U/L, （7720±3554） pmol/L and （94. 22±29. 79） ng/L respectively, strikingly higher than those of control group [ （ 101.18±17. 10）μg/ L, （19.57±9.02） U/L, （3140.00±632.00） pmol/L, （47.70±15.08） ng/L,P〈O. 05]. However, no significant difference existed in the OPG serum level between these two groups （ P 〉 0. 05 ）. Conclusion Iron overload could inhibit osteogenesis and enhance bone-resorption, which might be the pathological mechanism of osteoporosis induced by iron overload.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第2期312-314,共3页
Chinese Journal of Experimental Surgery
基金
江苏省自然科学基金资助项目（BK2008165）
教育部博士点专项基金资助项目（20103201110020）
苏州大学附属第二医院青年职工预研基金资助项目（SDFEYQNl105）
关键词
铁过载
骨质疏松
骨转换
活体成像系统
Iron overload
Osteoporosis
Bone turnover
In vivo imaging system
