摘要
目的观察联合激动Toll样受体（TLRs）的树突细胞对小鼠结肠癌的杀伤作用以及荷瘤小鼠的生存时间。方法用转染人癌胚抗原（CEA）的MC38小鼠结肠癌细胞株（MC38．CEA）和MC38细胞株（1X10^6个／只）皮下注射人CEA转基因小鼠，7d后将不同的树突状细胞（DC）疫苗（1x10。个／只）于对侧皮下注射小鼠，每周2次测量各组小鼠肿瘤的垂直直径，并记录小鼠的生存时间，绘制各组肿瘤生长曲线和小鼠生存曲线。结果在预防性疫苗干预的MC38一CEA肿瘤模型中，第41天联合TLR且荷载CEA肽段的DC（TLR．DC＋CEA）组小鼠肿瘤平均体积（1091．20±226．12）mm。明显小于其余各组（P〈0．01），并且存活期明显优于其余各组（P〈0．01）；在治疗性疫苗干预时，第41天TLR—DC＋CEA组小鼠肿瘤平均体积（6487．20±1024．47）mill。，小于磷酸盐缓冲液（PBS）对照组（9361．20．4-2654．64）mrn’（P〈0．05），而与其余对照组差异无统计学意义（P〉0．05）；在小鼠MC38肿瘤模型中，各组肿瘤平均体积差异无统计学意义（P〉0．05），且各组小鼠存活期差异无统计学意义（P〉0．05）。结论联合激动TLR的Dc疫苗能抑制表达CEA的小鼠结肠癌生长并延长小鼠生存期。
Objective To observe the anti-tumor effect of dentritic cells （DC） vaccine activated by combined Toll-like receptor （TLR） on murine colon cancer model and the survival time of tumor-bearing mice. Methods Murine colon cancers for the experiment were established using MC38-CEA and MC38 cells （ 1× 10^6 cells per mouse）. Seven days later, different DC vaccines （ 1× 10^6cells per mouse） were injected subcutaneously. The growth status of the subcutaneous tumors was observed twice per week and the survival was recorded. Finally the mean tumor volume in each group was analyzed for plotted curves and survival curves of mice in each group were depicted. Results In the MC38-CEA murine tumor model with preventive DC vaccine, mean tumor volume in the group of TLR activated DC pulsed with CEA peptide [ （1091.20 ＋ 226. 12） mm3] was significantly smaller than that of other groups （P 〈 O. 01 ） and the sur- vival was significantly longer （P 〈 O. 01 ） ; When with therapeutic DC vaccine, the mean tumor volume of TLR ＋ CEA-DC group [ （ 6487. 20 -＋ 776. 18 ） mm3 ] on the day 41 was smaller than that of PBS group [ （ 9361.20 _＋ 2654. 64） mm3 ] （ P 〈 0.05 ）, but there was no difference from other groups （ P 〉 0. 05 ）. In the murine tumor model with MC38 colon cancer cells, mean tumor volume in all groups had no significant difference （ P 〉 0. 05 ） , and the survival time in each group had no significant difference （ P 〉 0. 05 ）. Con- elusion Combined TLR activated DC vaccine can inhibit the growth of MC38-CEA colon cancer cells and prolong survival days.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第2期181-183,共3页
Chinese Journal of Experimental Surgery
基金
上海市科委基金资助项目（09411960700）
关键词
结肠癌
树突细胞
TOLL样受体
Colon carcinoma
Dendritic cells
Toll-like receptor
