摘要
本研究应用荧光分光光度计,通过对水华鱼腥藻活体荧光光谱的扫描,确定了蓝藻藻蓝蛋白荧光测定的最佳荧光激发波长为620nm,荧光发射波长为650nm,建立了水华鱼腥生物量与藻蓝蛋白特征荧光强度的线性检测关系,表明应用荧光光谱法检测藻蓝蛋白荧光强度反应蓝藻生物量具有特异性和可行性,该方法具有简便、快速、操作简单、样品无需处理等优点。为测定蓝藻生物量提供了新的检测方法。
Anabaena flos-aquae were scanned in vivo by fluorescence spectrum.The estimated optimal excitation and emission wavelength of cyanobacterial phycocyanin fluorescence were 620nm,650nm respectively and a linear relationship between anabaena flos-aquae biomass and the phycocyanin characteristic fluorescence intensity was detected.The results impressed that applying fluorescence spectrum to detect the phycocyanin fluorescence intensity and reflect the anabaena flos-aquae biomass is specific and feasible.The method is characterized by its simplicity,high speed,simple operation and impretreatment.A fresh method is provided to detect cyanobacterial biomass in water.
出处
《现代科学仪器》
2011年第6期111-113,共3页
Modern Scientific Instruments
基金
科技部国际合作项目(2007DFA90660)
重庆市重大科技攻关项目(CSTC
2006AA7003)
关键词
荧光光谱法
水华鱼腥藻
藻蓝蛋白
蓝藻
Fluorescence spectrometry
Anabaena flos-aquae
Phycocyanin
Cyanobacteria
