摘要
目的:探索蒙药复方菖蒲四味两种提取物体外对人肝癌细胞(SMMC-7721)的生长影响。方法:体外培养条件下,分别用不同浓度的蒙药复方菖蒲四味乙醇和石油醚提取物处理人肝癌细胞(SMMC-7721),采用噻唑蓝(MTT)法测定细胞的活性。结果:蒙药复方菖蒲四味的两种提取物对体外培养的SMMC-7721细胞在12.5～200μg/mL剂量内均具有剂量依赖性的抑制作用,与空白对照组比较,差异显著(P<0.01);阳性对照药环磷酰胺亦可显著地抑制SMMC-7721细胞的增殖。体外增殖抑制作用的强弱为石油醚提取物样品2>乙醇提取物样品1。结论:两种提取物对SMMC-7721细胞具有明显的抑制作用,有进一步研究的潜在价值。
Objective：To study the effects of two extracts from Mongolian Medicine Changpu siwei on Human Hepatoma cells（SMMC-7721） in vitro.Method：With different concentrations of two extracts from Mongolian Medicine Changpu siwei deal with Human Hepatoma cells（SMMC-7721）in vitro culture conditions.MTT was used to observe the effect.Results：The two extracts has a dose-dependent inhibiting effect on Human Hepatoma cells（SMMC-7721） in vitro,and compared with the control group the difference is significant（P0.01）;The Positive control Cyclophosphamide also has a inhibiting effect on Human Hepatoma cells（SMMC-7721）,inhibitation the rate of the samp le2 is higher than sample1.Conclusion：The two extracts has a clear inhibiting effect on Human hepatoma cells（SMMC-7721）and has further research potential value.
出处
《内蒙古医学院学报》
2011年第6期495-498,共4页
Acta Academiae Medicinae Neimongol
基金
内蒙古自治区高等学校科学研究项目(NJ10137)
呼和浩特市科技计划项目(2010)
内蒙古自治区重大科技计划项目(2009)
关键词
蒙药复方菖蒲四味
噻唑蓝法
抗癌活性
人肝癌细胞
Mongolian Medicine Changpu siwei
MTT assay
Anticancer activity
Human Hepatoma cells
