摘要
利用已公布的水稻OsCRY2基因序列设计引物,扩增部分基因片段,成功构建了ihpRNA植物表达载体pSC1301-347-OsCRY2,并通过农杆菌介导法将OsCRY2干扰片段导入水稻获得了转基因植株。根据转基因植株主要农艺性状的表现,分析了该基因的功能。结果表明,抑制OsCRY2基因表达会强烈延迟水稻开花与成熟;转基因植株穗与剑叶变短,结实率下降,粒增长,其他农艺性状未见明显变化。
According to the published rice OsCRY2 gene sequence,we designed the related primers to obtain part of the gene by PCR and constructed an ihpRNA plant expression vector named pSC1301-347-OsCRY2.The OsCRY2 gene interference fragment was then introduced into rice by Agrobacterium-mediated transformation.The functions of the gene was preliminarily analyzed by the performance of the transformants.The results indicated that the transgenic plants in which the expression of OsCRY2 gene was suppressed give rise to both retarded flowering and maturing.Moreover,the transgenic plants have short panicles and flag leaves,lower seed-set percent with the grain becoming longer while the other agronomic traits do not change apparently.
出处
《核农学报》
CAS
CSCD
北大核心
2011年第6期1094-1099,共6页
Journal of Nuclear Agricultural Sciences
基金
福建省科技计划重点项目(2008I01021117)
国家高技术研究发展计划("863计划")项目(2007AA10Z181)
