摘要
在水稻中花11的辐射诱变库中,筛选到1个深绿色突变体(ZM1120)。该突变体的茎、叶颜色较野生型对照深(绿);在播种后第60、90天,突变体的叶绿素a含量比野生型分别高16.0%和7.2%,类胡萝卜素含量比野生型分别高23.1%和24.2%,播种后第90天突变体的净光合速率和蒸腾速率比野生型分别高16.3%和11.4%;该突变体的各种农艺性状发生显著变化,其中株高、剑叶长、剑叶宽、每株分蘖数、穗长及结实率等显著下降,但是粒长、千粒重分别增加了7.9%和2.6%。对突变体与野生型杂交F2代群体的遗传分析表明,该突变表型受1对隐性核基因控制,进一步的基因克隆及功能分析将对阐明光合作用机制和水稻增产育种具有重要的理论意义和应用价值。
A deep green rice mutant ZM1120 was screened from the γ-rays irradiation mutation library of Zhonghua 11.Compared to the wild-type control,this mutant were darker（greener） in shoots and leaves,and after sowing 60 and 90d,the content of chlorophyll were increased by 16.0% and 7.2%,respectively,and the content of carotenoid also increased by 23.1% and 24.2%,respectively.After sowing 90d the net photosynthetic rate and transpiration rate were increased by 16.3% and 11.4%,respectively.The agronomical traits of this mutant were significantly changed,and the traits of plant height,flag-leaf length,flag-leaf width,tiller number per plant,panicle length and setting rate decreased,but the grain length and 1000-grain weight increased by 7.9% and 2.6%.Genetic analysis revealed that the mutation phenotype was controlled by a single recessive nuclear gene,and further cloning and function assay will be useful for understanding the mechanism of photosynthesis and for rice breeding in future.
出处
《核农学报》
CAS
CSCD
北大核心
2011年第6期1072-1076,1163,共6页
Journal of Nuclear Agricultural Sciences
基金
国家重大科技专项(2011ZX08009-003-001)
国家自然科学基金项目(31171519
31101130)
浙江省自然科学基金项目(Y3110234
Y3100531)
浙江省重点科技创新团队(2010R50024)
浙江省公益性技术应用研究计划项目(2011C22005)
水稻生物学国家重点实验室(State Key Laboratory of Rice Biology)开放项目
关键词
水稻
高叶绿素
突变体
表型
遗传分析
rice
high chlorophyll content
mutant
phenotype
genetic assay
