摘要
目的探讨脂联素基因＋45T／G单核苷酸多态性与汉族男性早发冠心病的相关性。方法将423例汉族男性患者（〈55岁）冠状动脉造影结果分为冠心病组（358例）及对照组（65例），采用PCR—RFLP分析脂联素基因＋45T／G单核苷酸多态性并检测脂联素及血脂、血糖等相关临床指标。结果冠心病组TG／GG基因型比例为51．1％，对照组TG／GG基因型比例为35．4％，差异有统计学意义（X2=5．45，P=0．022）。矫正其他冠心病易感因素后，Logistic回归发现＋45T／G单核苷酸多态性与汉族男性早发冠心病发生相关（OR=1．843，P=0．035，95％CI：1．045—3．250）。结论脂联素基因＋45T／G单核苷酸多态性与汉族男性早发冠心病发生相关，TG／GG基因型可能为汉族男性早发冠心病易感基因型。
Objective To examine the relationship between single nucleotide polymorphism （SNP） of adiponectin （APN） locus ＋ 45T/G and Han male patients with premature coronary artery heart disease （pCAD）. Methods A total of 423 male patients of Han ethnic group （ 〈55 yr old） undergoing coronary arteriography were recruited. Among them, 358 patients were diagnosed as pCAD while another 65 normal control （NC）. All subjects were genotyped for adiponectin gene SNP ＋ 45 by polymerase chain reaction- restriction fragment length polymorphism （PCR-RFLP） assay. Results Statistical differences of TG/GG genotype ratio were observed between two groups. The TG/GG genotype ratio in the pCAD group was 51. 1% versus 35.4% in the NC group （X2 = 5.45, P = 0. 022）. After an adjustment of conventional risk factors, such as age, body mass index （BMI） and hypertension, the adiponectin gene SNP ＋ 45 TG/GG genotype was strongly associated with Han ethnic group male pCAD by binary logistic regression analysis （ OR = 1. 843, P =0. 035, 95% （21： 1. 045 - 3.250）. And there were statistical differences between T＇F genotype and TG/GG genotype among pCAD subjects in the following factors： BMI, total cholesterol （TC）, low-density lipoprotein-C （LDL-C） and APN level （P 〈 0. 05）. By Pearson correlation analysis, APN was negatively correlated with TC, LDL-C and SNP ＋ 45T/G polymorphism. Conclusion The SNP of adiponectin locus ＋ 45T/G is associated with male pCAD. And TG/GG genotype is a possible predisposing gene for Han ethnic group male pCAD.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2011年第48期3413-3416,共4页
National Medical Journal of China
基金
山东省自然科学基金（Y2006C130）
关键词
脂联素
冠状动脉疾病
多态性
单核苷酸
Adiponeetin
Coronary disease
Polymorphism, single nucleotide
