摘要
目的:分析miR-224在胰腺癌组织中的表达,探讨其在胰腺癌细胞增殖、细胞周期及凋亡中的意义.方法:采用TagMan MGB探针法定量分析40例原发性胰腺癌及对应的癌旁组织MiR-224的表达;利用反义技术降低胰腺癌细胞(Aspc-1和Bxpc-3)中miR-224的表达;采用MTT比色法检测细胞增殖的改变,利用流式细胞技术检测胰腺癌细胞周期和凋亡情况.结果:在40例胰腺癌病例中,43%(17/40)的胰腺癌组织miR-224表达明显高于癌旁组织(P<0.05);反义miR-224转染胰腺癌细胞Aspc-1和Bxpc-3后,miR-224的表达明显降低,Aspc-1和Bxpc-3胰腺癌细胞生长受到明显抑制,其生长主要停滞在G0/G1期,而S期和G2/M期细胞的比例下降;另外降低miR-224的表达,Aspc-1和Bxpc-3胰腺癌细胞早期凋亡明显增加.结论:miR-224在胰腺癌组织中表达上调,降低其表达能明显抑制Aspc-1和Bxpc-3细胞的生长和诱导细胞早期凋亡增加,miR-224有可能成为胰腺癌基因表达调控的新靶点.
AIM： To investigate the expression of miR-224 in pancreatic carcinoma and to evaluate the role of miR-224 in pancreatic cancer cell proliferation and apoptosis. METHODS： The expression of miR-224 in 40 pancreatic carcinoma tissue specimens and matched tumor-adjacent nontumorous tissue specimens was detected by real-time fluorescence PCR. After using the antisense technology to decrease the expression of miR-224 in pancreatic cancer cells （Aspc-1 and Bxpc-3）, MTT assayand flow cytometry were performed to inves- tigate the impact of miR-224 down-regulation on cell proliferation, cell cycle progression and apoptosis. RESULTS： MiR-224 was found to be overexpressed in 43% of pancreatic carcinoma cases （P 0.05）. After antisense microRNA-mediated knockdown of miR-224, the proliferation of Aspc-1 and Bxpc-3 cells was significantly inhibited. Aspc-1 and Bxpc-3 cells were mainly arrested in G0/G1 phase, and the percentage of cells in S and G2/M phases decreased. In addi- tion, miR-224 knockdown in Aspc-1 and Bxpc-3 cells resulted in an increase in early apoptosis. CONCLUSION： MiR-224 is overexpressed in human pancreatic carcinoma. Inhibition of miR-224 expression can not only effectively suppress growth but also induce cell apoptosis of Aspc-1 and Bxpc-3 cells. MiR-224 may serve as a new molecular target for the treatment of pancreatic carcinoma.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第33期3409-3414,共6页
World Chinese Journal of Digestology
基金
江西省科技厅基金资助项目
No.2009ZDS13700
关键词
胰腺癌
MiR-224
反义单核苷酸
Pancreatic carcinoma
MiR-224
Anti- sense oligonucleotides
