摘要
目的研究青蒿琥酯对佐剂性关节炎大鼠的抗炎免疫机制。方法建立佐剂性关节炎大鼠模型,随机分为空白组、AA模型组、甲氨蝶呤阳性药物对照组和青蒿琥酯大、中、小剂量组(20.0,10.0,2.5 mg.kg.d-1),并灌胃给药。ELISA法检测各组对单核细胞趋化因子(MCP-1)、调节活化T细胞表达和分泌因子(RANTES)和肿瘤坏死因子(TNF-α)的影响。结果青蒿琥酯大、中、小剂量组能显著降低血清TNF-α,与模型组比较差异有统计学意义(P<0.05);青蒿琥酯小剂量组与其大、中剂量组或者与甲氨蝶呤组比较差异有统计学意义(P<0.05);青蒿琥酯各剂量组均能显著降低血清中MCP-l和RANTES的表达,与模型组比较差异有统计学意义(P<0.05);而青蒿琥酯小剂量组与大、中剂量组或者与甲氨蝶呤组比较差异有统计学意义(P<0.05)。结论青蒿琥酯抗炎和免疫调节作用可能是与抑制炎症因子相关。
Objective To study the mechanisms of artesunate on anti-inflammatory of adjuvant arthritis（AA） in rats.Methods Eighty clean grade male Wistar rats were selected to model adjuvant arthritis.Randomly divided into 6 groups（n=10）： including the blank group,AA modle group,methotrexate（MTX）group,large,moderate and small dose artesunate groups（20.0,10.0,2.5 mg·kg·d-1）.The effect of cytokines and chemokines on CIA was measured by ELISA.Results Artesunate significantly reduced the expression of tumor necrosis factor α（TNF-α） compared with the model groups.The difference was significantly different（P0.05）.And small dose of artesunate group significantly reduced the expression of TNF-α compared with large,moderate dose of artesunate groups and MTX group.Artesunate could significantly reduced the expression of macrophage chemoattractant protein 1α（MCP-1）,chemokine activation normal T cell expressed and secreted（RANTES）and in serum of AA rats（P0.05）.Conclusion The mechanism of anti-inflammatory action of artesunate may be relate to the inhibition of inflammatory factors.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2012年第1期46-48,共3页
The Chinese Journal of Clinical Pharmacology
基金
国家自然科学基金资助项目(81160376)
桂林市攻关课题基金资助项目(20090538)
关键词
佐剂性关节炎
青蒿琥酯
单核细胞趋化因子
调节活化T细胞表达和分泌因子
肿瘤坏死因子
adjuvant arthritis
artesunate
macrophage chemoattractant protein 1α
chemokine activation normal T cell expressed and secreted
tumor necrosis factor α
