摘要
目的研究前列腺癌组织中6种miRNAs的表达及其意义,并探讨它们在前列腺癌诊断中的应用价值。方法采用核酸分子原位杂交(in situ hybridizationI,SH)技术,结合组织微阵列平台分别检测38例良性前列腺增生(benign prostate hyperpla-sia,BPH)和52例前列腺癌(prostatic cancer,PCa)中6种miRNAs的表达情况。结果 6种miRNAs在PCa中的表达率与BPH比较差异均有统计学意义(P<0.05)。6种miRNAs的表达均与PCa的Gleason评分相关(P<0.05);与患者的年龄及血清PSA水平均无明显相关性(P>0.05)。miR-15b、miR-182和miR-96的表达与PCa的临床分期相关(P<0.05);miR-96的表达与肿瘤累及前列腺叶数相关(P<0.05)。结论 miRNAs与PCa的发生、发展以及生物学行为有关,并可能作为PCa诊断及判断预后的生物标记物。
Purpose To investigate the expression of 6 individual miRNAs and their significance in prostate cancer, and to discuss their use for the diagnosis of prostate cancer. Methods In situ hybridization staining combined with tissue microarray technology for 6 miRNAs was performed in 38 samples of benign prostate hyperplasia and 52 samples of prostate cancer. Results The expression of all 6 miRNAs showed a significant difference between the prostate cancer and benign prostatic hyperplasia tissues （ P 〈 0. 05 ）. The positive rate of these 6 miRNAs was significantly related to the Gleason grade of prostate cancer（ P 〈 0. 05 ） , but not with age and serous PSA level of the patient （P 〉 0. 05 ）. On the otherhand, the expression of miR-15b , miR-182 and miR-96 correlated with tumor clinical stage （ P 〈 0. 05 ）, The expression of miR-96 correlated with the extension of tumor invasion in prostate （ P 〈 0. 05 ）. Conclusions The miRNAs may be involved in the development of prostate cancer, and they may be important biomarkers for diagnosis and prognostic assessment of nrostate cancer.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2012年第1期50-53,共4页
Chinese Journal of Clinical and Experimental Pathology
关键词
前列腺肿瘤
前列腺增生
MIRNAS
原位杂交
prostate neoplasms
prostatic hyperplasia
microRNAs
in situ hybridization
