摘要
本研究以光温敏核不育系P88S为受体材料,用农杆菌法进行转化,并对获得的转基因植株进行分子鉴定及性状鉴定。结果显示:Bar基因和Cry1Ca基因在水稻P88S中成功整合并表达。光温敏核不育系P88S经过转基因后育性发生改变,表现出一定的可育度。转基因后P88S的谷粒宽及千粒重与对照之间的差异达到了极显著水平。
The rice variety P88S, a photoperiod-sensitive and/or thermo-sensitive genie male sterile （PTGMS） line, wastransformed by Agrobacterium-mediated transformation system, and the transformants were demonstrated by molecularidentification and trait assessment. The result showed that the Bar and CtylCa genes were successful integrated in ricegenome and expressed. But the pollen sterility of transformed P88S showed some degree of fertility after geneticallymodified when that of P88S was perfect sterile. And there were also significant difference at the 1% level betweentransgenic lines and P88S in grain width and 1000-grain weight.
出处
《农业现代化研究》
CSCD
北大核心
2012年第1期104-107,共4页
Research of Agricultural Modernization
基金
转基因生物新品种培育科技重大专项资助项目(编号:2011ZX08001-003)
湖南省科技重大专项资助项目(编号:2009FJ1004)
关键词
水稻
光温敏核不育系
Cry1Ca基因
BAR基因
抗除草剂
抗虫
rice
photoperiod-sensitive and/or thermo-sensitive genic male sterile line
CrylCa gene
Bar gene
herbicide resistance
insect resistance
