摘要
哺乳动物Sirtuins家族目前共发现7个成员:SIRT1～SIRT7,它们均为NAD+依赖性且从细菌到人类都保守的一类酶.人们已经对这7种去乙酰化酶进行了亚细胞定位.目前,对其研究主要集中在对细胞发育相关的重要转录因子如p53、FOXO家族及相关蛋白的去乙酰化修饰.Sirtuins对许多生理过程有着重要的调节作用,尤其是当发现它们对寿命延长的调控作用后,Sirtuins引起了人们极大的关注,且都发表在世界顶级刊物上.聚ADP核糖聚合酶(poly ADP-ribose polymerase,PARP)是一类存在于大多数真核细胞中的蛋白质翻译后修饰酶,尤其是聚ADP核糖聚合酶1(PARP-1)在细胞内DNA损伤修复等过程中起着重要作用,该酶同样以NAD+作为催化反应的底物.有研究发现,Sirtuins家族成员与PARP-1在细胞内某些重要生理过程中存在着相互作用.本文评述了Sirtuins家族成员、PARP-1的生物学特点,并就其参与哺乳动物细胞凋亡的调控机制和相关信号通路进行了详细的论述,以期对Sirtuins家族成员、PARP-1生物学功能及其相互作用的研究提供理论指导.
The silent information regulator（SIR） genes（Sirtuins） are highly conserved NAD＋-dependent enzymes from bacteria to mammals,which include seven family members as SIRT1 to SIRT7 with defined subcellular localizations.Sirtuins are actively involved in the regulation of gene expression and cellular development with their histone deacetylase activity to modulate transcription factors like p53 and FOXO etc.Sirtuins have also been implicated for key roles in certain physiological processes,especially linked to lifespan expansion as being attentioned.PARP-1 is a member of poly ADP-ribose polymerase（PARP） family,which is also comprised of NAD＋-dependent enzymes and play vital roles in multiple cellular processes,such as DNA repair and maintenance of the genomic integrity.As the interplay of sirtuins and PARP-1 in cellular development has been extensively reported,this article reviews the biological characteristics of the sirtuins family members,and their functions with PARP-1 in the pathways and the process of cell apoptosis.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2012年第1期9-17,共9页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家自然科学基金项目(No.31172206)~~
