摘要
目的 探讨神经母细胞瘤组织中胚胎干细胞基网Oct-4的表达情况及其意义。方法收集39例神经母细胞瘤和相应癌旁组织，应用实时定量PCR和Western印迹法检测Oct-4的表达，并分析其表达与临床病理参数的相关性。结果术前未化疗的神经母细胞瘤Oct-4的mRNA相对表达量为6．53±1．74，明显强于瘤旁组织1．04±0．65，两组间差异有统计学意义（P〈0．05）；其中Ⅲ～Ⅳ期肿瘤Oct-4mRNA相对表达量8．81±0．67较Ⅰ～Ⅱ期肿瘤4．26±0．54高，差异有统计学意义（P〈0．05）。Ⅲ～Ⅳ期肿瘤中术前未化疗的Oct4mRNA相对表达量8．81±0．67明显高于术前化疗的肿瘤3．15±0．42（P＜0．05）。神经母细胞瘤Oct-4表达与患儿性别、年龄、肿瘤大小、肿瘤部位、病理分型等参数无相关性。结论Oct-4可能与神经母细胞瘤的发生、发展相关，化疗药物的使用对其表达有抑制作用。
Objective To investigate the expression ot Oct-4 m neuroblastoma. Metnods i ne Oct 4 expression in 39 neuroblastoma tissues and paracancerous tissues was determined by real-time PCR and Western blot. The relationship between the expression of Oct-4 and clinical data was also analyzed. Results The expression level of Oct-4 in preoperative neuroblastoma tissues was significantly higher than that in paracancerous tissues（P〈0. 05）, and its expression in stage Ⅲ-Ⅳ neuroblastoma was higher than that in stage Ⅰ - Ⅱ. In tumors of stage Ⅲ-Ⅳ, the Oct-4 expression was significantly decreased in the subgroup with preoperative chemotherapy when compared with that without preoperative chemotherapy. Conclusions The expression of Oct-4 correlates positively with the progression of neuroblastoma. The Oct-4 expression can be inhibited by chemotherapy,
出处
《中华小儿外科杂志》
CSCD
北大核心
2012年第1期1-4,共4页
Chinese Journal of Pediatric Surgery
基金
国家自然科学基金资助项目（编号：30801198）
