摘要
目的以腺病毒介导连环蛋白P120(P120ctn)基因,转染人肝癌SMMC-7721细胞,观察其对SMMC-7721细胞侵袭能力的影响。方法用腺病毒介导的P120ctn基因转染SMMC-7721细胞,用逆转录-PCR(RT-PCR)和Western Blot检测细胞中P120ctn表达水平,并测定转染后细胞侵袭能力的变化。结果腺病毒介导的P120ctn转染SMMC-7721后,P120ctn的表达水平显著上升;体外侵袭实验显示转染后SMMC-7721的侵袭能力明显下降。结论腺病毒介导的P120ctn基因能够在SMMC-7721细胞中有效表达,并显著抑制肝癌细胞的侵袭能力。
Objective To study the influence of P120ctn gene on invasiveness of human hepatoma cell line SMMC-7721. Methods Recombinant adenoviruses containing human wild-type P120ctn cDNA（AdP120ctn） was infected into SMMC-7721 lines. The mRNA and protein expressions of Pl20ctn were detected with RT-PCR and Western Blot,respectively. And Boyden Chamber were undertaken to assay the change of invasion of transfected cell lines in vitro. Results Gene and protein expressions of P120ctn were up-regulated when SMMC-7721 was transinfected with AdP120ctn. In vitro invasion of SMMC-7721 cells was significantly inhibited after infected with AdP120ctn. Conclusion P120ctn gene mediated by adenovirus can express efficiently in SMMC-7721 cells and decrease the invasiveness of the cells significantly.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第3期275-276,278,F0003,共4页
Chongqing medicine
基金
重庆市自然科学基金资助项目(2009JJ1298)
