摘要
目的利用抗体芯片分析氯喹对小鼠巨噬细胞RAW264.7经细菌内毒素(LPS)刺激后炎性细胞因子分泌的影响。方法体外培养单核-吞噬细胞系RAW264.7,用生物素分别标记从空白组、LPS处理组、氯喹处理组及氯喹加LPS处理组细胞中提取的总蛋白,然后与预先点有40个主要炎症细胞因子的特异性抗体的抗体芯片膜反应,目标蛋白与辣根过氧化物酶(HRP)标记的抗链霉素生物素抗体结合并曝光显示反应信号,最后用激光扫描仪将其转化为图像文件进行分析。结果与LPS处理组比较,氯喹加LPS处理组炎症细胞因子的表达呈现低表达。结论氯喹对LPS诱导的RAW264.7细胞炎症因子的分泌具有抑制作用。
Objective To investigate the effects of chlorquine on inflammatory cytokines secretion from mouse macrophages by antibody chips. Methods Proteins from the RAW 264.7 cells in LPS group,chlorquine group,both chlorquine and LPS group,and a control group were labeled with biotin. The biotin-labeled proteins reacted with antibody chips on which the antibodies of 40 major inflammatory cytokines were prepared. The target proteins were conjugated with streptomycin antibody labeled with horseradish peroxidase（HRP） ,and signals were imaged by laser scanner. Results Compared with the LPS group,the levels of inflammatory cy tokines of chlorquine group were notably decreased. Conclusion Antibody chips demonstrate chlorquine can significant inhibit in flammatory cytokines secretion from mouse macrophages indused by LPS.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第3期214-215,219,共3页
Chongqing medicine
