摘要
目的:观察血红素加氧酶-1在高糖诱导的心肌细胞肥大中的表达,以及异丙酚对其表达的影响。方法:体外培养乳鼠心肌细胞,分组给药后,用BCA法测心肌细胞的蛋白质含量;用计算机图像分析系统检测心肌细胞表面积;用DCFH-DA活性氧检测试剂盒检测细胞内氧自由基的产生;用RT-PCR和Western blot分别检测心肌细胞中HO-1mRNA及蛋白表达。结果:高糖组心肌细胞表面积、细胞蛋白含量及氧自由基产生均升高。50μmol/L浓度的异丙酚对高糖诱导的肥大心肌细胞表面积、细胞蛋白含量及氧自由基的产生均有显著的抑制作用,而给予HO-1抑制剂后其作用明显减弱。结论:HO-1可能参与了异丙酚抑制心肌细胞肥大的作用。
Objective： To investigate the expression of heme oxygenase-1（HO-1） in high glucose-induced hypertrophic cardiomyocytes,and to study the effect of propofol on HO-1 system which is a possible new cytoprotective pathway of propofol.Methods： Primary cultured neonatal rat cardiomyocytes were exposed to a high concentration of glucose（HG,25.5 mmol/L） or normal glucose（5.5 mmol/L,control）,HG in the presence of the HO-1 inducer cobalt protoporphyrin（CoPP）（10 μmol/L）,the HO-1 inhibitor zinc protoporphyrin（ZnPP）（10 μmol/L）,propofol（50 μmol/L）,propofol plus ZnPP or CoPP plus ZnPP,respectively,for 48 hours.Myocyte cross-sectional area was measured by immunocytochemical analysis.Myocyte protein content was determined by bicinchoninic acid assay.Reactive oxygen species（ROS） were detected by fluroescence of DCFA-DA staining.Reverse transcription polymerase chain reaction（RT-PCR） and Western blot analysis were used to detect HO-1 gene and protein expression.Results： Cardiomyocyte hypertrophy was induced by HG,and manifest as significantly increased cardiomyocyte cross-sectional area and enhanced protein production（all P0.01,versus control）.Cardiomyocyte hypertrophy was accompanied by increased ROS.CoPP and propofol,respectively,significantly increased HO-1 expression and attenuated HG-mediated cardiomyocyte hypertrophy,and reduced ROS production（P0.05 or P0.01）.These protective effects of propofol or CoPP were abolished by ZnPP.Conclusion： Propofol ameliorates hyperglycemia induced cardiomyocyte hypertrophy,at least in part,by increasing HO-1 expression.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2012年第1期32-35,58,共5页
Medical Journal of Wuhan University
基金
国家自然科学基金资助项目(编号:30872426)
关键词
血红素加氧酶-1
高糖
心肌
异丙酚
Heme oxygenase-1
High glucose
Cardiomyocytes
Propofol
