摘要
目的研究prohibitin在H2O2致心肌细胞氧化应激损伤中的表达及中药丹参多糖对其调节作用。方法以H2O2干预体外培养乳鼠心肌细胞建立氧化应激心肌损伤模型;MTT法检测细胞活力;流式细胞术检测细胞凋亡率;免疫印迹法检测prohibitin蛋白表达情况。结果与模型组相比,丹参多糖组的细胞活力明显上升,凋亡率显著降低,其中高剂量组尤为明显(P<0.01);prohibitin在正常心肌细胞内表达较低,而在模型组表达代偿性增高,丹参多糖用药组其表达有所增加,高剂量组增加更明显,与模型组相比有显著性差异(P<0.05)。结论丹参多糖可通过上调prohibitin蛋白表达发挥其保护H2O2致心肌细胞损伤的作用。
Objective To study the expression of prohibitin in oxidative stress myocardial cells induced by H2O2 and regulation of Salvia polysaccharides on it. Methods Use H2O2 to stimulate the primary cultured myocardial cells to establish cellular oxidative stress model;to check cell viability by MTT assay;flow cytometry was used to detect cell apoptosis;the expression of prohibitin was detected by western boltting. Results Compared with the model group,Salvia polysaccharide groups significantly increased cell viability and decreased the apoptosis ratio,especially the high dose group（P0.01）;the expression of prohibitin was low in normal myocardial cells,while higher in the model group;SM polysaccharide treatment groups obviously increased its level,especially the high dose group;there was significantly difference compared with the model group（P0.05）. Conclusion Salvia polysaccharides can protect myocardial cell injury induced by H2O2 by up-regulating the expression of prohibitin.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2011年第12期2889-2891,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.30572435
No.81072776)
广州市白云区科技项目(No.2010-kz-39)
关键词
氧化应激
丹参多糖
心肌细胞
凋亡
Oxidative stress
Salvia polysaccharides
Myocardial cells
Apoptosis
