摘要
目的探讨柞树皮二氯甲烷提取物对脂多糖(LPS)和D-氨基半乳糖(D-GalN)诱导败血症休克小鼠的保护作用。方法 50只雄性ICR小鼠随机分为正常对照组,模型对照组,阳性对照组,高剂量给药组和低剂量给药组。两个剂量给药组分别按300,100 mg/kg腹腔注射柞树皮二氯甲烷提取物;阳性对照组按30 mg/kg腹腔注射地塞米松;正常对照组和模型对照组以等体积生理盐水灌胃,30 min后,除正常对照组外,其余各组均腹腔注射20μg/kg LPS和700 mg/kg D-GalN,比较各组血清门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)的活性和一氧化氮(NO)的含量,以及肝组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,同时每天观察小鼠的死亡率至第2天。结果柞树皮二氯甲烷提取物两个剂量给药组明显提高小鼠的存活率,显著抑制小鼠血清AST和ALT含量的升高(P<0.01),降低血清NO含量(P<0.01),同时明显升高小鼠肝组织SOD活性(P<0.05)、降低MDA含量(P<0.05)。结论柞树皮二氯甲烷提取物通过降低NO含量、肝保护作用,从而提高败血症性休克小鼠的存活率。
Objective To investigate the protective effect of dichloromethane extract of Quercus mongolica stem bark on lipopolysaccharide（LPS） and D-galactosamine（D-GalN） induced septic shock in mice. Methods Fifty male ICR mice were randomly divided into normal control group,model control group,positive control group,high-dosage group and low-dosage group.The drug groups were pretreated intraperitoneally with dichloromethane extracts of Q.mongolica stem bark or saline 30 min before intraperitoneally injection of LPS（20 μg/kg）/D-GalN（700 mg/kg）.Lethality rate was observed daily for up to 2 days.Liver damage was evaluated by measuring serum aspartate aminotransferase（AST） and alanine aminotransferase（ALT） activities.The serum level of nitric oxide（NO）,superoxide dismutase（SOD） activity and malondialdehyde（MDA） content in liver were also measured in each group above. Results Pretreatments of mice with the dichloromethane extracts of Q.mongolica stem bark at doses of 300 mg/kg and 100 mg/kg increased survival rates to 60% and 80%,respectively.As an initial mechanistic study,the AST and ALT activities,and the NO content in serum of mice pretreated with the dichloromethane extracts of Q.mongolica stem bark（300 mg/kg and 100 mg/kg） were significantly lower than those of control LPS/D-GalN treated group.The dichloromethane extracts of Q.mongolica stem bark significantly increased the activity of SOD,and decreased the content of MDA in liver tissue. Conclusion The dichloromethane extracts of Q.mongolica stem bark showed the protective effect on LPS/D-GalN induced lethality through down-regulation of NO and hepatoprotection.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2011年第12期2886-2887,共2页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.30760291)
关键词
柞树皮
败血症休克
死亡率
保护作用
Quercus mongolica stem bark
Septic shock
Lethality rate
Protective effect
