摘要
目的探讨骨桥蛋白(osteopontin,OPN)在正常宫颈、CIN及宫颈癌组织中的表达与宫颈癌发生、发展、侵袭和转移关系。方法采用免疫组织化学S-P法检测正常宫颈组织、CIN和宫颈癌组织中OPN的表达情况。结果 OPN在正常宫颈组织、CIN和宫颈癌中的阳性表达率呈上升趋势,三组比较有显著性差异(P<0.05);在CINⅠ组和CINⅡ-Ⅲ组中的阳性表达率呈上升趋势,但两组比较无显著性差异(P>0.05)。在宫颈癌组织中表达:OPN的阳性表达率随临床期别的增加、组织分化程度的降低而增高,差异有显著性;OPN在无淋巴结转移宫颈癌组织中的阳性表达率显著高于有淋巴结转移组(P<0.05)。结论OPN在组织中的阳性表达率由正常宫颈组织→CIN→宫颈癌呈递增趋势,表明了OPN在宫颈癌的发生、发展中发挥了重要的作用。OPN在宫颈癌组织中的表达与宫颈癌的临床分期、组织分化程度及淋巴结转移有一定的相关性,为OPN可望成为判断宫颈癌预后的肿瘤标志物及成为宫颈癌治疗的靶基因奠定基础。
Objective To explore the differences expression of OPN in normal cervical tissue,CIN, cervical cancer tissues and their relationship with cervical cancer occurrence, development and prognosis. Methods The expression of OPN in normal cervical tissue, CIN and cervical cancer tissue were detected by immunohistochemical technique, and their clinical significance were analyzed. Results The OPN positive expression rate were raised gradually from normal cervical tissues, CIN to cervical cancer and have significantly differences of three groups（P〈0.05）. That in CIN II -III is higher than in CIN I group,but two groups of comparisons was not statistically significant（P〉0.05）. In cerical cancer organization：OPN positive expression rate was heighten with clinical stage of cerical cancer increasing and histopatho logical differentiation degree reducing. The OPN positive expression rate in lymph node metastasis were significantly higher than without lymph node metastasis. Conclusion The OPN positive expression rate were increasing trend by normal cervical tissue-CIN I CIN II-III-cerical cancer. This showed OPN in cervical cancer occurrence, development plays an important role. Through testing the OPN expression in cerical cancer tissues,it has the important meaning on the judgement of cerical cancer malignant degree,prognosis. Make OPN may become judgment cerical cancer prognosis of the tumor markers.
出处
《中国实验诊断学》
2012年第1期65-67,共3页
Chinese Journal of Laboratory Diagnosis
关键词
宫颈癌
OPN
免疫组织化学
肿瘤标志物
cerical cancer
Osteopontin
immunohistochemical
tumor markers
