摘要
目的通过比较不同免疫状态的Balb/c鼠和裸鼠中枢神经免疫调节相关脑区功能的差异,探讨外周免疫系统释放的神经免疫调节网络上传信号的细胞来源。方法 Balb/c鼠和裸鼠各50只,每种动物随机分成免疫2、4、6、8d组和健康对照组。应用免疫组织化学技术检测不同免疫时间点动物的神经免疫调节相关脑区下丘脑外侧区(lateral hypothalamic area,LH)和杏仁核前区(anterior amygdaloid area,AA)功能活化信号白细胞介素-1β(IL-1β)的表达水平,分析T细胞功能缺陷对神经免疫调节功能的影响。结果与健康对照组比较,Balb/c鼠免疫2、4、6d组LH、AA脑区IL-1β表达明显增高(P<0.05),且免疫后2d其表达即增高,免疫后4d达高峰,免疫后6d开始下降(均P<0.05),免疫后8d其表达量降至基础生理水平(P>0.05)。裸鼠免疫组LH、AA脑区IL-1β表达趋势与Balb/c鼠结果相似。裸鼠免疫组LH、AA脑区IL-1β表达水平与Balb/c鼠比较无统计学差异(P>0.05)。结论 Balb/c鼠与裸鼠经抗原免疫注射后均可启动LH、AA脑区的神经免疫调节功能,提示免疫系统功能活化后,向中枢神经系统上传的神经免疫调节功能启动信号由树突状细胞和T辅助细胞共同提呈,但主要来自树突状细胞。
Objective To test the cellular sources of signals released by peripheral immune system and transformed by the neuroimmunoregulation network,and to compare functional differences in central nervous system（CNS） related to neuroimmune regulations between Balb/c mice and Balb/c-nu mice in different immune states.Methods Fifty Balb/c mice and fifty Balb/c-nu mice were randomly divided into the immune 2,4,6 and 8 days＇ groups and the control group（10 cases in each group）.Immunohistochemical techniques were used to observe expression levels of interleukin-1β（IL-1β） in encephalic regions of neuroimmune regulation [lateral hypothalamic area（LH） and anterior amygdaloid area（AA）] at different immune time.SPSS11.0 was used to analyze variance.The effect of T cells defect on neuroimmunoregulation was analyzed.Results The immunized Balb/c mice expressed more IL-1β in the LH,AA regions compared with the control group（P0.05）.The level of IL-1β increased on the 2nd day after immunization,and reached the peak on the 4th day,then started to decline on the 6th day（P0.05,respectively）and returned to physiological level on the 8th day（P0.05）.Balb/c-nu mice experienced the similar procedures as the Balb/c mice,while immunized Balb/c-nu mice expressed lower levels of IL-1β than immunized Balb/c mice at the same time point（P0.05）.Conclusions Balb/c mice and Balb/c-nu mice can both initiate neuroimmunoregulation responses in LH,AA areas after injected with antigens.Dendritic cells（DCs） and Th cells both present neuroimmunoregulation signals to CNS after activation of the immune system and this presentation mainly relies on DCs.
出处
《中国神经免疫学和神经病学杂志》
CAS
北大核心
2012年第1期37-39,44,共4页
Chinese Journal of Neuroimmunology and Neurology
基金
国家自然科学基金资助项目(81072489)
