摘要
目的建立测定替加氟片含量及其溶出度的高效液相色谱法。方法分析柱为惠普C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-乙腈-水(10∶5∶85),流速为1.0 mL/min,检测波长为271 nm。结果替加氟进样量在2.041～4 082 ng范围内与峰面积线性关系良好(r=0.999 9),平均回收率为99.56%,RSD=0.13%(n=9)。替加氟的检测限和定量限分别为1.4 ng和4.7 ng。结论所用方法简便、可靠、准确,可用于替加氟片的常规分析。
Objective To develop an HPLC method to determine the content and dissolution of Tegafur Tablets.Methods The C18 column（250 mm×4.6 mm,5 μm）was used as analytical column.Mobile phase consisted of methanol-acetonitrile-water（10 ∶5 ∶85）,flow rate was 1.0 mL/min.The detection wavelength was set at 271 nm.Results The standard curve was linear in the concentration range from 2.041-4 082 ng for tegafur（r=0.999 9）,the average recoveries of tegafur was 99.56%with RSD of 0.13%（n=9）.The limit of detection and quantitation for the assay were 1.4 and 4.7 ng,respetively.Conclusion The method is simple,accurate and sensitive,and can be used for the routine analysis of resveratrol.
出处
《中国药业》
CAS
2012年第1期32-33,共2页
China Pharmaceuticals
